Posters
« Back
Development of the Comprehensive Method for Steroid Analysis by GCxGC-HR-TOFMS
EP26892
Poster Title: Development of the Comprehensive Method for Steroid Analysis by GCxGC-HR-TOFMS
Submitted on 29 Jan 2018
Author(s): Jonelle Shiel, David Alonso, and Viatcheslav Artaev
Affiliations: LECO Corporation
This poster was presented at MSACL
Poster Views: 357
View poster »


Poster Information
Abstract: Identification and quantitation of steroid metabolites in biological samples are essential for screening for various hormonal disorders. Many of these metabolites are closely related isomers and cannot be easily separated by LC or one-dimensional GC due to their chemical and structural similarity, which complicates or even prevents reliable analyte assignment.[1] Comprehensive two-dimensional gas chromatography (GCxGC), coupled to a high resolution high mass accuracy time-of-flight mass spectrometer (HR-TOFMS) provides dramatically enhanced chromatographic separation, an increase in sensitivity, and reliable detection and identification of analytes of interest.

Thirty-three steroids from different classes (progestogens, androgens, estrogens, glucocorticoids, mineralocorticoids) were derivatized and analyzed using GCxGC coupled to HR-TOFMS to achieve detection and reliable identification in complex matrices.
Summary: -AML library was created by running individual derivatized steroid samples.
-GCxGC Method was developed allowing reliable separation of all 33 steroids in the sample.
-All 33 analytes were found in the mixture and positively assigned using accurate mass confirmation for molecular ions (when available) and major fragment ions, RI, and AML/NIST library matches.
-GCxGC-HR-TOFMS was successfully applied for non-targeted steroids identification in mixture.
References: References
1 Comprehensive Steroid Analysis By GCxGC-TOFMS, Michael Groessl et al, 288477, ThOG, Proceedings of the 65th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN, June 4-8, 2017.
2 https://www.leco.com/simply-gcxgc
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


PERFIL DE COMPONENTES VOLATILES DE MIELES FLORALES Y MONOFLORALES COLOMBIANAS
Salamanca,G. G. , Osorio, T. M.; Scriche, R. I.

MALDI Imaging Mass Spectrometry for the study of cardiovascular pathology
Takashi Nirasawa1, Megumi Terada2,3, Hiroko Namba2, Nobuto Kakuda2, Patrick Bruneval3, Hatsue Ishibashi-Ueda4 and Masaya Ikegawa1

Distinct deposition of amyloid-bspecies in brains with Alzheimer’s disease pathology visualized with MALDI imaging mass spectrometry
Nobuto Kakuda1, Tomohiro Miyasaka1, Takashi Nirasawa2, Shigeo Murayama3, Yasuo Ihara1 and Masaya Ikegawa1

Routine Analysis of Host Cell Proteins in Antibody Preparations using PASEF
Stuart Pengelley1, Guillaume Tremintin², Waltraud Evers1, Detlev Suckau1

A Novel Method For Discovery of Peripheral Blood Biomarkers in Idiopathic Pulmonary Fibrosis Using Extensive Depletion and TMTcalibratorTM Tissue-Enhanced Plasma Proteomics
I. Pike1, M. Bremang1, P.J. Wolters 2, R. Gaster3, S. Turner3, M. Decaris3