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DISPERSIVE SOLID-PHASE EXTRACTION AND IN-VIAL FILTRATION AS A SIMPLIFIED CLEAN-UP FOR ETHYLPHENOLS DETERMINATION IN RED WINES
EP25653
Poster Title: DISPERSIVE SOLID-PHASE EXTRACTION AND IN-VIAL FILTRATION AS A SIMPLIFIED CLEAN-UP FOR ETHYLPHENOLS DETERMINATION IN RED WINES
Submitted on 31 Mar 2017
Author(s): Ariel Fontana, Rubén Bottini
Affiliations: Laboratorio de Bioquímica Vegetal, IBAM-CONICET-UNCuyo, Mendoza, Argentina.
This poster was presented at PTTCON 2017
Poster Views: 731
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Poster Information
Abstract: [Brettanomyces] sp. is a microorganism related to several wine faults, most notably those known as phenolic off-flavor or Brett character. This flavor can be described as horsey, leathery, medicinal, smoky or savory being caused by the presence of ethylphenols (EPs). Although Brettanomyces is considered a spoilage organism that cause an objectionable flavor in red wine when their related spoilage compounds are present in high levels, low levels of EPs are sometimes considered adding complexity to a wine. Due to the relevant organoleptic impact of EPs on wine quality at variable levels, the development of analytical methodologies for the determination of them in wine samples is a subject of interest.
In this work, we report the development and validation of a new high-throughput analytical method to monitor EPs in red wines using a quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation followed by HPLC-UV determination. The approach combines in-vial filtration with dispersive solid-phase extraction (d-SPE) in a fast and convenient clean-up of QuEChERS extracts. During method development, different commercial sorbents for d-SPE were investigated and compared with respect to analyte recoveries. The method was validated at 0.1, 0.5 and 5 mg L-1 spiking levels, and the results for all analytes recoveries were between 72 and 119 % with RSD ≤ 15 %. The developed method was applied to several samples; being 4-ethylphenol and 4-vinylguaiacol the compounds detected with major frequency. The presented approach has high sample throughput and usefulness in screening studies for wines Brett character analysis.
Summary: We report the development and validation of a new high-throughput analytical method to monitor EPs in red wines using a quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation followed by HPLC-UV determination. The approach combines in-vial filtration with dispersive solid-phase extraction (d-SPE) in a fast and convenient clean-up of QuEChERS extracts.Report abuse »
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