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EP38495
Poster Title: Distribution of Antimicrobial Resistance Genes in Various Types of Food Including Meat, Produce (Vegetables and Fruits) and Dairy Products in Canada
Submitted on 17 Mar 2022
Author(s): Sohail Naushad, Chris Grenier, Beverley Phipps-Todd, Andrea Arzate, Karen Zhao, Nur Syifa Azmil, Dele Ogunremi and Hongsheng Huang
Affiliations: Ottawa Laboratory - Fallowfield, Canadian Food Inspection Agency
This poster was presented at 2021 European Symposium on Food Safety
Poster Views: 584
Submitted on 17 Mar 2022
Author(s): Sohail Naushad, Chris Grenier, Beverley Phipps-Todd, Andrea Arzate, Karen Zhao, Nur Syifa Azmil, Dele Ogunremi and Hongsheng Huang
Affiliations: Ottawa Laboratory - Fallowfield, Canadian Food Inspection Agency
This poster was presented at 2021 European Symposium on Food Safety
Poster Views: 584
Abstract: Introduction: Distribution of microorganisms with antimicrobial resistance (AMR) genes in food including meat, produce (vegetables and fruits) and dairy products has been recognized as a growing public health concern worldwide. Food contaminated with microorganisms are responsible for numerous foodborne illnesses, including outbreaks caused by AMR gene-containing bacteria.
Purpose: This study is aimed at determining the distribution of AMR genes in various commodities including meat, produce and dairy products for a better understanding of the associated hazards and facilitating quantitative risk assessment.
Methods: Food samples (n = 59), including meat (n = 18), produce (n = 30, vegetables = 21, fruits = 9) and dairy (n = 15), were randomly purchased at different times from local retails. Microbes in food were enriched (24 h at 37°C) using modified Schaedler media, followed by genomic DNA extraction and detection of 87 AMR genes relevant to human, animal and agriculture using a commercial AMR gene qPCR array kit (Qiagen).
Results: Thirty-three AMR genes had at least one occurrence in all three groups with potential resistance against aminoglycoside, fluoroquinolone, tetracycline, macrolide and Class C beta-lactam. Eight AMR genes including erythromycin resistance genes were not detected in any of the food samples. The meat contained the highest number of AMR genes/sample (mean = 14, ranged from 2 to 28), followed by produce (mean = 10, 1 to 34) and dairy products (mean = 7, 1 to 23). Four AMR genes including vanB (vancomycin resistance) were only detected in meat samples, 12 and 4 other AMR genes were only detected in produce and dairy products, respectively. The mecA gene, encoding a methicillin-resistant Staphylococcus aureus-specific penicillin-binding protein, was also detected in produce and dairy products but not in meat.Summary: The results in this study provide useful baseline data on AMR gene presence in food in Canada and indicate that different types of food may acquire microorganisms containing certain AMR genes from different sourcesReferences: Dione et al. 2016. A quasi-universal medium to break the aerobic/anaerobic bacterial culture dichotomy in clinical microbiology. Clin Microbiol Infect. 22:53-58.
Purpose: This study is aimed at determining the distribution of AMR genes in various commodities including meat, produce and dairy products for a better understanding of the associated hazards and facilitating quantitative risk assessment.
Methods: Food samples (n = 59), including meat (n = 18), produce (n = 30, vegetables = 21, fruits = 9) and dairy (n = 15), were randomly purchased at different times from local retails. Microbes in food were enriched (24 h at 37°C) using modified Schaedler media, followed by genomic DNA extraction and detection of 87 AMR genes relevant to human, animal and agriculture using a commercial AMR gene qPCR array kit (Qiagen).
Results: Thirty-three AMR genes had at least one occurrence in all three groups with potential resistance against aminoglycoside, fluoroquinolone, tetracycline, macrolide and Class C beta-lactam. Eight AMR genes including erythromycin resistance genes were not detected in any of the food samples. The meat contained the highest number of AMR genes/sample (mean = 14, ranged from 2 to 28), followed by produce (mean = 10, 1 to 34) and dairy products (mean = 7, 1 to 23). Four AMR genes including vanB (vancomycin resistance) were only detected in meat samples, 12 and 4 other AMR genes were only detected in produce and dairy products, respectively. The mecA gene, encoding a methicillin-resistant Staphylococcus aureus-specific penicillin-binding protein, was also detected in produce and dairy products but not in meat.Summary: The results in this study provide useful baseline data on AMR gene presence in food in Canada and indicate that different types of food may acquire microorganisms containing certain AMR genes from different sourcesReferences: Dione et al. 2016. A quasi-universal medium to break the aerobic/anaerobic bacterial culture dichotomy in clinical microbiology. Clin Microbiol Infect. 22:53-58.
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