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Distribution of Resistance-Associated Variants (RAVs) in HCV Genotypes (GT) 1a and 1b Populations.
EP24559
Poster Title: Distribution of Resistance-Associated Variants (RAVs) in HCV Genotypes (GT) 1a and 1b Populations.
Submitted on 04 Oct 2016
Author(s): Elian Rakhmanaliev1, Pramila Ariyaratne1, Zhang Rui1, Kok Siong Poon2, Cui Wen Chua2, Mui Joo Khoo2, Evelyn S. Koay2, Ekawat Passomsub3, Wasun Chantratita3, Gerd Michel1
Affiliations: 1Vela Diagnostics Pte. Ltd., Singapore 2Molecular Diagnosis Centre, Department of Laboratory Medicine, National University Hospital, Singapore 3Department of Pathology, Faculty of Medicine, Ramathibodi Hospital Mahidol University, Bangkok, Thailand
This poster was presented at 25th Annual Conference of the Asian Pacific Association for the Study of the Liver , Tokyo, Japan, Feb, 2016
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Poster Information
Abstract: Antiviral drugs in combination with interferon and ribavirin significantly improve virologic response rate in patients with GT1 chronic hepatitis C. One of the most important considerations is the potential development of RAVs. Timely detection and reporting of RAVs and HCV GTs is critical for drug regiment and can minimize the development of resistance to antiviral drugs.
We used a newly developed automated Next Generation Sequencing (NGS)-based integrated workflow, comprised of a robotic liquid handling system, kits for RNA extraction and library preparation (Sentosa SQ HCV Genotyping Assay), Ion Torrent based deep sequencing system and bioinformatics analysis and reporting software. The data reports on GTs 1a and 1b include 136 known RAVs in NS3, NS5A and NS5B genes. However, the system does not make direct treatment recommendations, which are left to investigators.
This study included 56 GT1a and 54 GT1b samples. 52.7%(58/110) of HCV strains were carrying 1 or multiple RAVs in 23 positions in all target genes. An unequal distribution of 4 mutations across the GT1 subtypes was observed. Frequency of Q80K mutation (NS3) was 25%(14/56) in GT1a and 1.9%(1/56) in GT1b. While mutations Q54H and Y93H (NS5A) were prevalent in GT1b: 42.6%(23/56) and 18.5%(10/56) respectively. Frequency of Q54H in GT1a population was 0%(0/56) and Y93H – 1.8%(1/56). Mutation V499A in NS5B gene was also prevalent in GT1b: 0%(0/56) in GT1a and 25.9%(14/56) in GT1b populations.
Simultaneous RAVs detection and GTs determination by one diagnostics tool provides complete, essential and important information for HCV treatment approaches.
Summary: Distribution of Resistance-Associated Variants (RAVs) in HCV Genotypes (GT) 1a and 1b Populations.Report abuse »
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