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DNA-free Platinum Taq DNA polymerase for reliable microbiome studies
EP29884
Poster Title: DNA-free Platinum Taq DNA polymerase for reliable microbiome studies
Submitted on 27 Mar 2019
Author(s): Kęstutis Bargaila, Vytautas Budrys, Andrius Krasauskas, Dovilė Lisauskienė, Milda Romeikaitė, Sonata Jurėnaitė-Urbanavičienė,Ramunė Leipuvienė and Juozas Šiurkus
Affiliations: Thermo Fisher Scientific
Poster Views: 454
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Poster Information
Abstract: Residual bacterial DNA in most commercial PCR enzymes poses challenges in analysis of microbial genomes, such as accurate detection of bacterial strains by 16S rRNA gene sequences. Here, we introduce Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free — a
PCR enzyme with the lowest DNA contamination levels as compared to competing “DNA-free” enzymes. Manufactured using a closed single-use system and stringent quality control, the DNA-free Platinum™ Taq DNA Polymerase offers minimized risk of DNA contamination in the enzyme while retaining the performance specifications associated Platinum™ hot-start technology. The purity of this enzyme offers clean backgrounds in reagents-only (or “negative”) controls of broad-range PCR and improves confidence in microbiome studies.
Summary: Residual bacterial DNA in most commercial PCR enzymes poses challenges in analysis of microbial genomes, such as accurate detection of bacterial strains by 16S rRNA gene sequences. Manufactured using a closed single-use system and stringent quality control, the DNA-free Invitrogen™ Platinum™ Taq DNA Polymerase is the ideal choice for microbiome applications where contaminating DNA can’t be tolerated.References: 1. Goodrich JK, Di Rienzi SC, Poole AC et al. (2014) Conducting a Microbiome Study. Cell 158
2. Salter SJ, Cox MJ, Turek EM et al. (2014) Reagent and laboratory contamination can critically impact sequence-based microbiome analyses. BMC Biology 12:87
3. Eisenhofer R, Minich JJ, Marotz C, Cooper A, Knight R, Weyrich LS (2019) Contamination in Low Microbial Biomass Microbiome Studies: Issues and Recommendations. Trends Microbiol 27(2)
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