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dPEG® Conjugates Significantly Increase Hydrodynamic Volume of Proteins
Poster Title: dPEG® Conjugates Significantly Increase Hydrodynamic Volume of Proteins
Submitted on 23 Jun 2014
Author(s): Quanta BioDesign
Affiliations: Quanta BioDesign
This poster was presented at World ADC Summit 2014
Poster Views: 2,033
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Poster Information
Abstract: In the therapeutic/diagnostic field, the advantage of increased apparent molecular weight allows proteins to appear larger in vivo, thus prolonging the half-life of the protein in the circulatory system as well as preventing it from being cleared through the kidneys as quickly as smaller proteins. This results in reduced dosing frequencies. Other advantages of conjugating PEG to proteins are lower incidences of side effects and decreased chances for immunogenicity. An enormous range of benefits to protein-based medicine through the application of PEGylation is already in published studies, and it is not difficult to envision further extension of known benefits and development of new benefits through PEGylation. This series of experiments was designed to demonstrate that discrete PEGs, when conjugated to proteins, increase the apparent molecular weight of the protein (presumably through increased hydrodynamic volume of the PEGylated protein). Both linear and branched dPEG® constructs were used with purified BSA as a model protein to demonstrate this occurrence. Data obtained from size exclusion chromatography was compared to MALDI-MS determined molecular weights to demonstrate a trend of increasing dPEG® construct size yielding increasing hydrodynamic volumes.
Summary: The term dPEG® is an acronym for “discrete polyethylene glycol” or “discrete PEG” and refers to a unique class of PEGs, having a unique, specific, single molecular weight, synthesized de novo, from pure, small units (e.g., triethylene glycol or tetraethylene glycol). These dPEG® compounds differ dramatically from long chain PEG polymers, which are made through polymeric processes and have variable chain lengths and consequently, variable polydispersity values.References: Report abuse »
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