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Edman Degradation, Peptide Mapping and De Novo Protein Sequencing
EP32739
Poster Title: Edman Degradation, Peptide Mapping and De Novo Protein Sequencing
Submitted on 23 Jun 2020
Author(s): Creative Proteomics
Affiliations: Creative Proteomics
This poster was presented at https://www.creative-proteomics.com/pdf/Protein-Sequencing.pdf
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Abstract: Edman Degradation is one of the N-terminal amino acid sequence analysis methods for peptide chains/proteins sequencing. The protein is reacted with PITC under weakly basic conditions, and then treated with an acid to free the amino terminal residue of the peptide chain in the form of PTH AA for subsequent analysis.Summary: Edman Degradation is one of the N-terminal amino acid sequence analysis methods for peptide chains/proteins sequencing. The protein is reacted with PITC under weakly basic conditions, and then treated with an acid to free the amino terminal residue of the peptide chain in the form of PTH AA for subsequent analysis.Report abuse »
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