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Effects of chronic intermittent hypoxia/reoxygenation  on body weight and glucose metabolism in rats
EP36145
Poster Title: Effects of chronic intermittent hypoxia/reoxygenation on body weight and glucose metabolism in rats
Submitted on 14 May 2021
Author(s): JIN Huanhuan
Affiliations: The First Affiliated Hospital of Inner Mongolia University of Science and Technology
This poster was presented at THE THIRDLY CONGRESS OF ASIAN SOCIETY OF SLEEP MEDICINE
Poster Views: 140
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Poster Information
Abstract: Abstract: Objective: To establish a rat model of intermittent hypoxia to observe the changes in body weight and glucose metabolism of rats under chronic intermittent hypoxia/reoxygenation environment. Methods :According to the random number table method, 24 healthy male SD rats, weighing about 200±10 g, were divided into control group (NC group) and intermittent hypoxia group (CIH group), each with 12 rats, suitable for environmental feeding, free diet. The CIH group rats were placed in an intermittent hypoxic chamber, and nitrogen was first circulated into the oxygen chamber for 25 seconds to gradually reduce the oxygen concentration in the chamber from 21% to the minimum oxygen concentration of 7%-8%, maintaining for 10 seconds, and then filling Air for 55 seconds to gradually restore the concentration in the oxygen chamber to 21% and then maintain it for another 30 seconds to maintain each cycle for 120 seconds. The rats will experience intermittent hypoxia events at 30 times/hour. The exposure time is from 9:00 am to pm daily At 5:00 (8 hours in total), a total of 12 weeks. During the intermittent hypoxia, the rats were not given food, and the rats were placed in a breeding cage during the rest of the time. They were kept normally and maintained at room temperature and air. From the 13th week to the 16th week, it was placed in a normal air environment for reoxygenation intervention. During the experiment, in order to remove the excess water and carbon dioxide in the oxygen chamber, an appropriate amount of quicklime was added to make it absorbed. The rats in the NC group were not exposed to hypoxia and were kept in normal air to maintain a suitable living environment. The rest of the conditions were the same as those in the CIH group. Before being placed in the oxygen chamber (that is, 0 weeks) and every 2 weeks after being placed in the oxygen chamber, the rats in the two groups were fasted with water for 8 hours and their body weights were measured. After the end of the 12-week intermittent hypoxia, the rats in the CIH group They were kept in normal air for 4 weeks, and their body weight was measured in the same way every week. The intracanthal venous blood of rats was drawn every 2 weeks to detect FBG and FINS, and HOMA-IR was evaluated according to the steady-state model. Results: At week 0, there was no difference in body weight between the two groups of rats (NC group vs. CIH group: 226.25±28.55 vs. 224.58±33.93, P>0.05). After 2 weeks of intermittent hypoxia, the weight of the CIH group was significantly lower than that of the NC group ( Compared with the CIH group in the NC group: 300.13±30.94 vs 254.58±35.82, P<0.05). By the end of the 6th week, the weight of the CIH group was still lower than that of the NC group. At the 10th and 12th weeks, the weight of the two groups tended to balance. After reoxygenation, the CIH group weighed more than the NC group. At 2 to 4 weeks of reoxygenation, the weight of the CIH group was significantly higher than that of the NC group (2 weeks of reoxygenation in the NC group compared to the CIH group: 458.75±52.01 vs 497.50±21.93, P< 0.05; 3 weeks of reoxygenation in NC group compared with CIH group: 449.00±50.09 vs 497.25±25.18, P<0.05; 4 weeks of reoxygenation in NC group compared with CIH group: 450.25±50.99 vs 502.75±23.21, P<0.05.) Under intermittent hypoxia, the FBG level of the CIH group was significantly higher than that of the NC group at the 8th weekend, and the difference was statistically significant (p<0.05); the CIH group FINS and HOMA-IR levels were significantly higher at the 4th, 6th and 8th weekend In the NC group, the difference was statistically significant (p<0.05); after reoxygenation intervention, the FBG level of the CIH group was higher at the first weekend of reoxygenation than the NC group, and the difference was statistically significant (p<0.05); second and third At the end of the 4th week, the FBG levels of CIH group and NC group were similar, and the difference was not statistically significant (p>0.05); the FINS levels of CIH group and NC group were similar, and the difference was not statistically significant (p>0.05); the HOMA-IR level of CIH group was at Reoxygenation was higher in the first and second weekends than in the NC group, and the difference was statistically significant (p<0.05); the levels of HOMA-IR at the third and fourth weekends were similar, and the difference was not statistically significant (p>0.05). Conclusion: At the initial stage of intermittent hypoxia, the weight of rats in the CIH group is lower than that in the NC group. Chronic intermittent hypoxia may inhibit the weight gain of rats; after the rats are adapted to hypoxia, the weight of the rats gradually returns to normal growth, and the rats reach hypoxia tolerance Status: After reoxygenation, the inhibitory effect on the weight gain of rats was lifted. The weight of the rats in the CIH group was much higher than that in the NC group, and the weight showed retaliatory growth. Intermittent hypoxia may affect the metabolism and growth of the rats. CIH increased the levels of FBG, FINS and HOMA-IR in rats and developed insulin resistance, indicating that CIH is involved in the development of insulin resistance in rats. After reoxygenation, insulin resistance in rats was reduced and the glucose metabolism disorder caused by OSAHS was improved.Summary: Effects of chronic intermittent hypoxia/reoxygenation
on body weight and glucose metabolism in rats
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