Posters
« Back
efficient crystallisation of a tuberculosis drug target using a dragonfly® crystal screen optimiser.
EP31197
Poster Title: efficient crystallisation of a tuberculosis drug target using a dragonfly® crystal screen optimiser.
Submitted on 10 Jan 2020
Author(s): Joby Jenkins1, Gary Cochrane1, David Smith1, Michal Blaszczyk2
Affiliations: SPT Labtech Ltd, Melbourn Science Park, Melbourn, Rosyton, Hertfordshire, SG8 6HB, UK, 2Biochemistry Dept., University of Cambridge, Cambridge
Poster Views: 27
View poster »


Poster Information
Abstract: Protein crystal optimisation is vital to ensure high quality X-ray diffraction data for the solving of high resolution structures. This process involves the set-up of a series of complex screening combinations where the ratios of the individual components identified from primary crystallisation studies are varied.
In order to reduce the effort and tedium of this process, SPTLabtech have designed dragonfly® crystal for crystallisation screening as an addition to their successful mosquito®liquid handling portfolio.
Enzymes that are essential for the growth of Mycobacterium tuberculosis (Mtb, the bacterium that causes tuberculosis) are valuable drug targets. During the hit validation step of drug discovery, there is a requirement for structure determination of the target. However crystallisation of the hit protein continues to remain the bottle neck of this process sometimes taking many months to achieve a suitable crystal.
The use of reliable, low volume automated systems such as SPTLabtech’s mosquito crystal has improved the time and accuracy of primary screening.
This poster will describe how Dr.Michal Blaszczykat Cambridge University, UK has used dragonfly crystal to optimise the conditions for the crystallisation of target enzymes involved in the growth of Mtb.
Summary: This poster demonstrates how Dr.Blaszczyk increased the efficiency of his optimisation process and obtained better diffracting crystals with the use of dragonfly crystal screen optimiser. Efficiencies were gained in time, money and sample volumes used. Improvements in accuracy and precision were also gained from using SPTLabtech’s automated liquid handlers.References: Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


dragonfly® crystal: an ideal solution to problematic liquids in screening environments
Joby Jenkins, Gillian Lewis

strategies for high-throughput ligand screening -automated co-crystallisation and soaking
Paul Thaw1, David Hargreaves2, JörgBenz3, Joby Jenkins1

Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A

In vitro selection & validation of synthetic single-domain antibodies & applications
Stéphanie Blachon1, Sandrine Moutel2, Selma Djander1&2, Vincent Collura1, Alexis Arrial1, Aurélien Olichon4, Franck Perez3, Jean-Christophe Rain1

Development of Tetrazole Based inhibitor against multple classes of Carbapenemases
Afroza Akhtar