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efficient crystallisation of a tuberculosis drug target using a dragonfly® crystal screen optimiser.
Poster Title: efficient crystallisation of a tuberculosis drug target using a dragonfly® crystal screen optimiser.
Submitted on 10 Jan 2020
Author(s): Joby Jenkins1, Gary Cochrane1, David Smith1, Michal Blaszczyk2
Affiliations: SPT Labtech Ltd, Melbourn Science Park, Melbourn, Rosyton, Hertfordshire, SG8 6HB, UK, 2Biochemistry Dept., University of Cambridge, Cambridge
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Poster Information
Abstract: Protein crystal optimisation is vital to ensure high quality X-ray diffraction data for the solving of high resolution structures. This process involves the set-up of a series of complex screening combinations where the ratios of the individual components identified from primary crystallisation studies are varied.
In order to reduce the effort and tedium of this process, SPTLabtech have designed dragonfly® crystal for crystallisation screening as an addition to their successful mosquito®liquid handling portfolio.
Enzymes that are essential for the growth of Mycobacterium tuberculosis (Mtb, the bacterium that causes tuberculosis) are valuable drug targets. During the hit validation step of drug discovery, there is a requirement for structure determination of the target. However crystallisation of the hit protein continues to remain the bottle neck of this process sometimes taking many months to achieve a suitable crystal.
The use of reliable, low volume automated systems such as SPTLabtech’s mosquito crystal has improved the time and accuracy of primary screening.
This poster will describe how Dr.Michal Blaszczykat Cambridge University, UK has used dragonfly crystal to optimise the conditions for the crystallisation of target enzymes involved in the growth of Mtb.
Summary: This poster demonstrates how Dr.Blaszczyk increased the efficiency of his optimisation process and obtained better diffracting crystals with the use of dragonfly crystal screen optimiser. Efficiencies were gained in time, money and sample volumes used. Improvements in accuracy and precision were also gained from using SPTLabtech’s automated liquid handlers.References: Report abuse »
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