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Electrochemical detection of microRNA using labelled DNA modified gold coated magnetic nanoparticles as dispersible electrodes
Electrochemical detection of microRNA using labelled DNA modified gold coated magnetic nanoparticles as dispersible electrodes
Submitted on 07 Apr 2017

Saimon Moraes Silva, Roya Tavallaie, David Brynn Hibbert, Richard D. Tilley, J. Justin Gooding
School of Chemistry, Australian Centre for NanoMedicine, ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales
This poster was presented at Pittcon Conference and Expo 2017
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Poster Abstract
Immobilization of single strands of DNA on a solid surface and subsequent hybridization with the complementary sequence are the foundation of many devices such as DNA biosensors, nanostructures, gene chips, DNA computers and therapeutic devices. A considerable number of reports employing surface hybridization reactions, in which sequence-specific recognition occurs between immobilized and solution nucleic acids are presented in the literature, although hybridization is not completely understood in bulk solution. The present work aims to provide a fundamental hybridization/electrochemical understanding of redox-DNA strands by using a methylene blue labelled probe DNA modified gold electrode and square wave voltammetry as the excitation/measurement technique. After modification of the gold surface, the methylene blue at probe DNA distal end is in close position to the underlying electrode in such way that electrons can be transferred easily. Upon hybridization with the target ferrocene labelled DNA a more rigid duplex is obtained decreasing the apparent rate of electron transfer between methylene blue and gold electrode. Additionally, as the target DNA contains a ferrocene label at its distal end, a second electrochemical process will occur related to the ferrocene redox reaction. An unusual regime is observed in which small faradaic currents for the ferrocene labelled target DNA are obtained, while the signal suppression for the probe label signal is obtained as expected. A method for the calculation of the surface coverage and rate constant is provided, which can be used for monitoring the DNA hybridization.Report abuse »
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