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Eliminating the need for Matrix-matched Calibration Standards for GC and LC Pesticide Residue Analyses of QuEChERS Extracts using a Robotic Solid Phase Extraction Clean-up Procedure.
EP21802
Poster Title: Eliminating the need for Matrix-matched Calibration Standards for GC and LC Pesticide Residue Analyses of QuEChERS Extracts using a Robotic Solid Phase Extraction Clean-up Procedure.
Submitted on 04 Apr 2014
Author(s): Bruce Morris, Richard Schriner, Rick Youngblood and Kim Gamble
Affiliations: Hill Laboratories, ITSP Solutions
This poster was presented at Pittcon 2014
Poster Views: 2,833
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Poster Information
Abstract: Since its introduction in 2003, QuEChERS extraction has been adopted by many laboratories undertaking pesticide residue analyses on samples from fruit and vegetables to complex herbs and spices. Clean-up of extracts for the removal of matrix components that interfere with subsequent LC-MS/MS or GC-MS/MS analyses has mostly relied on dispersive solid-phase extraction (dSPE). However, for many matrices, co-extractants causing suppression or enhancement of analyte signals remain after dSPE, requiring the use of matrix-matched calibration standards, or spike additions, for accurate quantitation. Precipitation of oils can also occur, with addition of aqueous buffer for LC-MS/MS, taking non-polar analytes out of solution. Column SPE has been employed for more complex sample types, as it offers improved clean-up. This is labor intensive and requires skilled technicians, therefore a novel robotic SPE clean-up was developed, using miniaturized cartridges. This can be placed on top of the instrument, and clean-up incorporated into the analytical run, saving time and labor. Experiments with a variety of sample types showed effective removal of interfering sugars, fatty acids, colors (chlorophyll and carotenoids), and oils for GC-MS/MS or LC-MS/MS analyses, with excellent recoveries of pesticide spikes presented. This has enabled use of solvent standards for calibration, and removed oil precipitation, allowing multiple sample types in one analytical run, with reduced instrument fouling, improving reproducibility over many injections.Summary: Use of a novel, miniaturized, robotized, SPE clean-up of QuEChERS extracts is presented, with LC-MS/MS spike recovery results for approximately 400 pesticides from four spice and food coloring samples. References: Report abuse »
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