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Enhancement of proliferation in a novel rat hepatocyte co-culture model after mitogenic stimulation
EP22560
Poster Title: Enhancement of proliferation in a novel rat hepatocyte co-culture model after mitogenic stimulation
Submitted on 12 Dec 2014
Author(s): Susan Hester, Michael A. McVay, Oyinade Adefuye, Alan Tennant, and Salman Khetani
Affiliations: USEPA
This poster was presented at 2012 SOT
Poster Views: 1,437
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Poster Information
Abstract: Primary mouse and rat hepatocyte cultures have long been the gold standard for assessment of cellular changes associated with chemical exposure. While helpful for assessing proliferative and apoptotic responses in vitro, these cultures have limitations due to loss of cells beyond 48hrs incubation. Rat HepatoPac™ was used to extend the culture time beyond 3 days and coupled with downstream quantitative high content imaging (HCI) technology for a more accurate assessment of the cell proliferative response following mitogenic stimulation.Summary: Primary mouse and rat hepatocyte cultures have limitations due to loss of cells beyond 48hrs incubation. Rat HepatoPac™ was used to extend the culture time beyond 3 days and coupled with downstream quantitative high content imaging (HCI) technology for a more accurate assessment of the cell proliferative response following mitogenic stimulation.References: *Ross, J. Human Constitutive Androstane Receptor (CAR) and Pregnane X
Receptor (PXR) Support the Hypertrophic but not the Hyperplastic
Response to the Murine Nongenotoxic Hepatocarcinogens
Phenobarbital and Chlordane In Vivo. TOXICOLOGICAL SCIENCES 116(2), 452–466 (2010)
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