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EP38440
Poster Title: Enhancing metabolic phenotyping by increased sensitivity provided by a vacuum insulated probe heated ESI (VIP-HESI) source
Submitted on 09 Mar 2022
Author(s): Melvin Gay1; Aude-Claire Morillon2; Sven Myers3; Magdalene Reinkensmeier3; Aiko Barsch3; Nicola Gray2; Berin Boughton2; Nathan Lawler2; Jeremy Nicholson2,4; Luke Whiley2
Affiliations: 1 Bruker Pty Ltd, Victoria, Australia 2 Australian National Phenome Centre, Health Futures Institute, Murdoch University, Australia 3 Bruker Daltonics GmbH & Co. KG Bremen, Germany 4 Institute of Global Health Innovation, Imperial College London, UK
Poster Views: 376
Submitted on 09 Mar 2022
Author(s): Melvin Gay1; Aude-Claire Morillon2; Sven Myers3; Magdalene Reinkensmeier3; Aiko Barsch3; Nicola Gray2; Berin Boughton2; Nathan Lawler2; Jeremy Nicholson2,4; Luke Whiley2
Affiliations: 1 Bruker Pty Ltd, Victoria, Australia 2 Australian National Phenome Centre, Health Futures Institute, Murdoch University, Australia 3 Bruker Daltonics GmbH & Co. KG Bremen, Germany 4 Institute of Global Health Innovation, Imperial College London, UK
Poster Views: 376
Abstract: Metabolic phenotyping is a highly informative approach in systems biology research that enriches the understanding of the metabolic mechanisms of infectious disease pathogenesis.
Advancements in quadrupole-time-of-flight (QToF) technology have enabled the use of high-resolution accurate mass instruments for both discovery and quantitative analyses [1]. However, metabolite concentrations can vary drastically between individuals and if sample volumes are limited, the dynamic range and sensitivity of the instrument become critical. Of significant influence to metabolite sensitivity is the design of the ionization source and efficient generation of charged ions.
Here, we determine if improvements in sensitivity and reproducibility occur in the newly developed ion source vacuum-insulated-probe heated electrospray ionization source (VIP-HESI) and compare the results to a previously validated method for detection 30 physiological biogenic amines on an Apollo II ESI source coupled to a timsTOF Pro.
Summary: Here, we determine if improvements in sensitivity and reproducibility occur in the newly developed ion source vacuum-insulated-probe heated electrospray ionization source (VIP-HESI) and compare the results to a previously validated method for detection 30 physiological biogenic amines on an Apollo II ESI source coupled to a timsTOF Pro.References: [1] https://doi.org/10.1016/j.talanta.2020.121872
Advancements in quadrupole-time-of-flight (QToF) technology have enabled the use of high-resolution accurate mass instruments for both discovery and quantitative analyses [1]. However, metabolite concentrations can vary drastically between individuals and if sample volumes are limited, the dynamic range and sensitivity of the instrument become critical. Of significant influence to metabolite sensitivity is the design of the ionization source and efficient generation of charged ions.
Here, we determine if improvements in sensitivity and reproducibility occur in the newly developed ion source vacuum-insulated-probe heated electrospray ionization source (VIP-HESI) and compare the results to a previously validated method for detection 30 physiological biogenic amines on an Apollo II ESI source coupled to a timsTOF Pro.
Summary: Here, we determine if improvements in sensitivity and reproducibility occur in the newly developed ion source vacuum-insulated-probe heated electrospray ionization source (VIP-HESI) and compare the results to a previously validated method for detection 30 physiological biogenic amines on an Apollo II ESI source coupled to a timsTOF Pro.References: [1] https://doi.org/10.1016/j.talanta.2020.121872
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