With new cfDNA NGS assays being able to detect variants from as little as 2-10ng DNA, assay validation to ensure sufficient accuracy has never been so critical. Reference materials that closely mimic real cfDNA samples are essential to support this effort. Here we present results from a comparative study into the production of cfDNA reference standards, assessing enzymatic fragmentation compared to fragmentation by sonication, and the benefit of including a size selection step.
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