Abstract: With new cfDNA NGS assays being able to detect variants from as little as 2-10ng DNA, assay validation to ensure sufficient accuracy has never been so critical. Reference materials that closely mimic real cfDNA samples are essential to support this effort. Here we present results from a comparative study into the production of cfDNA reference standards, assessing enzymatic fragmentation compared to fragmentation by sonication, and the benefit of including a size selection step.Summary: Liquid biopsies hold great promise to revolutionise the field of clinical oncology testing. cfDNA can be extracted from a routine patient blood sample and used to determine the genetic profile of a solid tumour located elsewhere within the body. This facilitates more informative disease management for the clinician, without the need for invasive surgery for the patient.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Single-cell studies: Focus on reverse transcription
Zucha Daniel, Androvic Peter, Kubista Mikael, Valihrach Lukas
Oral Soft Tissue Lumps and Bumps
Dr. Amal Hassan Zakarya, Sarah Ahmed Mohamed, Noura Mohamed Kamal, Heba Ahmed Saleh
Correlation between tissue expression of miRNA137 and CD8 in oral lichen planus
Sanaa aghbari, olfat shaker, shaimaa Omar, and el Rahman, atria attia
Endocrine Disruptors and Impact of Nutritional Intervention on Hypothyroid Women - A Study in Kolkata
Strategies for High-Titer Protein Expression Using the ExpiCHO and Expi293 Transient Expression Systems
Chao Yan Liu, Jian Liu, Wanhua Yan, Kyle Williston, Katy Irvin, Henry Chou, Jonathan Zmuda