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Evaluation of an automated SPE method for samples derived from capillary microsampling
Poster Title: Evaluation of an automated SPE method for samples derived from capillary microsampling
Submitted on 21 Nov 2013
Author(s): Paul-Gerhard Lassahn1, Petra Lombardi1, Winfried Wagner-Redeker1, Marie-Claude Robaudi-Fraschini2, Reto Bolliger3, Guenter Boehm3
Affiliations: 1 Swiss BioAnalytics AG, Basel, Switzerland; 2 Debiopharm International S.A., Lausanne, Switzerland; 3 CTC Analytics AG, Zwingen, Switzerland
This poster was presented at EBF 2013, Barcelona
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Abstract: • The automated workflow described gave equivalent results (LLOQ, precision, accuracy) compared to samples processed manually by protein precipitation. The overall time/sample is slightly higher for the automated protocol. However, since only minimal intervention is required, process safety is superior and human resources are freed up for other tasks.

• The automated protocol may be further optimized in several aspects. As shown a volume of 20 μL is sufficient for complete elution of the analyte from the cartridge. Figure 6 indicates that a loading volume of 2-5 μL diluted plasma is sufficient allowing the use of low-volume samples. Both aspects are important for further optimizing this protocol for analysis of samples originating from studies using microsampling techniques.

• Methods for the automated optimization of the SPE conditions exist and will be applied in future studies.
Summary: In this poster we describe an automated SPE procedure using small, single use cartridges in a modified PAL System liquid handler for the analysis of samples derived from capillary microsampling. Results are compared to results obtained from identical samples processed manually by protein precipitation.References: Nilsson LB, Ahnoff M, Jonsson O; Bioanalysis (2013) 5(6), 731–738Report abuse »
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