Posters
« Back
ExpiHO™: Surpassing the Performance of 293 in a Transient CHO Expression System
EP23339
Poster Title: ExpiHO™: Surpassing the Performance of 293 in a Transient CHO Expression System
Submitted on 02 Sep 2015
Author(s): Roland Leathers, Chao Yan Liu, Virginia Spencer, Shyam Kumar, Jian Liu, Ping Liu, Henry Chiou, Jonathan F. Zmuda
Affiliations: Thermo Fisher Scientific
This poster was presented at Drug Discovery 2015 ELRIG, Telford, UK
Poster Views: 1,923
View poster »


Poster Information
Abstract: CHO cells are the predominant host for biotherapeutic protein expression, with roughly 70% of licensed biologics manufactured in CHO. Multiple attributes make CHO cells desirable for bioproduction including the ability to adapt to high-density suspension culture in serum-free and chemically-defined media and the incorporation of post- translational modifications that are biologically-active in humans. For these reasons, the ability to produce transient CHO-derived proteins early on during drug development is highly advantageous to minimize, as much as possible, changes in protein quality/function observed when moving from R&D to bioproduction. Unfortunately, CHO cells express lower levels of protein than HEK293 cells in existing transient systems, in some instances 50-100 times less than the best 293-based systems, and only modest titer improvements are obtained through the optimization of individual components of existing transient CHO workflows. To address the significant unmet need for higher transient CHO protein titers, systems-based approaches were employed whereby the latest advances in cell culture media, feeds, transfection reagents and expression enhancers were optimized in conjunction with a new high-expressing CHO cell clone to generate a simple and robust workflow for transient protein expression in CHO cells capable of generating gram per liter protein titers in 10-14 days. These advances will allow for unprecedented access to CHO-derived proteins early on during candidate selection and may serve to revolutionize the use of CHO cells for transient protein expression during the drug development process. This novel transient CHO expression system is called ExpiCHO™Summary: The ExpiCHO™ CHO expression system was designed from the ground up using a systems-based approach whereby the latest advances in cell culture media, feeds, transfection reagents and expression enhancers were optimized in conjunction with a new high-expressing CHO cell clone to generate a simple and robust workflow for transient protein expression in CHO cells capable of generating gram per liter protein titers in 10-14 days (eg. human IgG's have been expressed in excess of 3g/L).References: Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Genetic Engineering in Male Sterility for Hybrid Variety Development
Abir Hasan Joy

VITVO: Mimicking In Vivo Complexity By The Innovative 3D Model
Olivia Candini1, Giulia Grisendi1, Elisabetta Manuela Foppiani1, Matteo Brogli1, Beatrice Aramini2, Valentina Masciale3, Carlotta Spano1, Tiziana Petrachi4, Elena Veronesi4, Pierfranco Conte5,6, Giorgio Mari1 & Massimo Dominici1,3

Digiceuticals
Helana Lutfi and Shaban Nuredini

Characterization of patient-derived organoids cultured on a gas-rich, liquid-liquid interface
James T. Shoemaker, Katherine R. Richardson, Jamie Arnst, Adam Marcus, Jelena Vukasinovic

Print Me An Organ?
Amanda Tan; Fatin Nur Syamimi Abdul Razak; Kathy Jia Qi Hu; Diew Fung Lau; Muhammad Hafizan Mustari; Nurul Fatihah Mohamad Azmi; Kar Hui Tan; Nithiyah Ramasundram