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ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
EP30832
Poster Title: ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
Submitted on 15 Oct 2019
Author(s): Kenneth Thompson, Maya Yovcheva, Sara Barnes, Melissa Cross, Katy Irvin, Natasha Lucki, Henry Chiou, and Jonathan Zmuda
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This poster was presented at PEACe (Protein Expression in Animal Cells) Conference, 2019
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Poster Information
Abstract: Unlike mammalian expression systems that have long since transitioned to serum-free, chemically defined culture media, relatively little innovation has taken place in insect expression systems, with insect cells continuing to rely on undefined, yeastolate-containing culture media that can exhibit significant cell growth and protein expression variability from lot-to-lot. Here, we describe the ExpiSf™ Expression System, the first chemically defined insect expression system that enables high-yield production of proteins and viral particles with consistent performance run after run using a fast, streamlined workflow. The system also enables faster baculovirus generation with an improved transfection reagent and a chemically defined enhancer to boost protein expression. We demonstrate scalability of the ExpiSf system from small to large scale (WAVE™ and Bioreactor) for the production and purification of recombinant proteins, virus-like particles (VLPs), and recombinant adenoassociated viruses (AAV). Together, the ExpiSf Expression System enables consistent production of diverse classes of recombinant macromolecules with significant improvements in titers compared to alternative BEVS platforms.Summary: The Baculovirus Expression Vector System (BEVS) is a versatile platform for expression of individual recombinant proteins as well as multimeric protein complexes, virus-like particles, and membrane proteins. Recently, BEVS has demonstrated particular utility in the commercial-scale manufacture of vaccines and gene therapy vectors. Here, we describe the ExpiSf™ Expression System, an insect expression system that enables high-yield production of proteins and viral particles. Report abuse »
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