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ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
EP31082
Poster Title: ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
Submitted on 10 Dec 2019
Author(s): Kenneth Thompson, Maya Yovcheva, Sara Barnes, Melissa Cross, Katy Irvin, Natasha Lucki, Henry Chiou, and Jonathan Zmuda
Affiliations: Thermo Fisher Scientific
Poster Views: 628
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Poster Information
Abstract: The Baculovirus Expression Vector System (BEVS) is a versatile platform for expression of individual recombinant proteins as well as multimeric protein complexes, virus-like particles, and membrane proteins. Recently, BEVS has demonstrated particular utility in the commercial-scale manufacture of vaccines and gene therapy vectors. Unlike mammalian expression systems that have long since transitioned to serum-free, chemically defined culture media, relatively little innovation has taken place in insect expression systems, with insect cells continuing to rely on undefined, yeastolate-containing culture media that can exhibit significant cell growth and protein expression variability from lotto-lot.Summary: Here, we describe the ExpiSf™ Expression System, the first chemically defined insect expression system that enables high-yield production of proteins and viral particles with consistent performance run after run using a fast, streamlined workflow. The system also enables faster baculovirus generation with an improved transfection reagent and a chemically defined enhancer to boost protein expression. Report abuse »
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