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FIA MRMS BASED METABOLOMICS REVEALS ANTI INFLAMMATORY EFFECTS OF HOP BITTER ACIDS IN DENDRITIC CELLS
EP38437
Poster Title: FIA MRMS BASED METABOLOMICS REVEALS ANTI INFLAMMATORY EFFECTS OF HOP BITTER ACIDS IN DENDRITIC CELLS
Submitted on 09 Mar 2022
Author(s): Emanuela Salviati 1 , Eduardo Sommella 1 , Giulio Verna 1 , Aiko Barsch 2 , Matthias Witt 2 , Jochen Friedrich 2 Pietro Campiglia 1
Affiliations: 1 University of Salerno, Department of Pharmacy, Fisciano , Italy; 2 Bruker Daltonics GmbH & Co. KG, Bremen, Germany
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Poster Information
Abstract: Inflammation is a complex, evolutionarily conserved process, that involves immune and non immune cells in the host for protection from harmful stimuli. Metabolic changes occur in macrophages and dendritic cells (DCs) leading to pro or anti inflammatory phenotypes In this regard, the growing field of immunometabolism aims to target specific metabolic pathways to modulate inflammation, and natural compounds are more and more used for preventive action 1. To understand the metabolic shift toward anti inflammatory phenotypes, metabolomics has emerged as leading approach. The metabolome profiling is usually carried out by liquid chromatography coupled to tandem mass spectrometry (LC MS/MS). As an alternative to LC MS/MS, direct infusion magnetic resonance mass spectrometry (DI MRMS) or flow injection (FIA) MRMS delivers short analysis time for sample screening and features unmatched mass accuracy, resolution, sensitivity, and isotopic fine structure. Hence, the FIA- or DI-MRMS workflows provide a higher throughput in metabolic profiling compared to LC MS methods 2. In this study, MRMS was used to highlight the metabolic changes in dendritic cells stimulated with lipopolysaccharide (LPS) and co treated with a Hop derived fraction rich in beta acids and prenylflavonoids and compared their effect with the flavonol quercetin.Summary: To understand the metabolic shift toward anti inflammatory phenotypes, metabolomics has emerged as leading approach. The metabolome profiling is usually carried out by liquid chromatography coupled to tandem mass spectrometry (LC MS/MS). References: [1] Immunometabolism: Cellular Metabolism Turns Immune Regulator. R. M. Loftus and D. K. Finlay, J. Biol. Chem. Chem., 2016 , 291, 1 10.
[2] Analysis of the metabolic switch induced by the spirulina peptide SP6 in high fat diet ApoE mice model : A direct infusion FT ICR MS based approach . E. Sommella , A. Carrizzo , F. Merciai , V. Di Sarno, D. Carbone, M. De Lucia, S. Musella , C. Vecchione and P. Campiglia , J. Pharm. Biomed. Anal., 2021 , 195,
[3] Toll like receptor induced changes in glycolytic metabolism regulate dendritic cell activation . C. M. Krawczyk, T. Holowka , J. Sun, J. Blagih , E. Amiel , R. J. DeBerardinis , J. R. Cross, E. Jung, C. B. Thompson, R. G. Jones and E. J. Pearce , Blood, 2010 , 115, 4742 4749.
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