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Gene Expression Profiling of Archived FFPE Samples
EP20647
Poster Title: Gene Expression Profiling of Archived FFPE Samples
Submitted on 20 Dec 2013
Author(s): Silvia Rüberg, Sabine Classen, Jana Ciomperlik, Dirk Dietrich, Ines Dischinger, Alena Böttcher, Sabrina Schmitz and Bernhard Gerstmayer
Affiliations: Miltenyi Biotech
Poster Views: 1,661
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Poster Information
Abstract: These samples represent a rich and valuable source that contains disease-relevant genetic and genomic information. However, molecular analysis of FFPE samples has been hampered not only due to difficulties in recovering cross-linked and partially degraded RNA from the respective tissues, but also because of the unavailability of suitable amplification and labeling protocols. In this study, total RNA from paired samples of FFPE and fresh frozen tissues was prepared. In addition, we performed biological and technical replicate experiments were performed and the robustness and reproducibility of different amplification and labeling protocols analyzed using samples that have been stored for different time periods.
Summary: According to the BBMRI (Biobanking and Biomolecular Resources Research Infrastructure) about 8,000,000 formalin-fixed paraffin-embedded (FFPE) samples derived from a multitude of different diseases have been collected in medical centers and biobanks all over Europe during the last decades.
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