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Graded organisation of fibronectin on polymer surfaces to tune cell response
EP23325
Poster Title: Graded organisation of fibronectin on polymer surfaces to tune cell response
Submitted on 01 Sep 2015
Author(s): E. Grigoriou, M. Cantini, M. J. Dalby, M. Salmeron-Sanchez
Affiliations:
This poster was presented at TCES annual conference 2015
Poster Views: 861
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Poster Information
Abstract: Cell behaviour is largely controlled by mechanochemical cues coming from the extracellular matrix (ECM). Fibronectin (FN), a major component of the ECM, binds to cell receptors and other ECM proteins and therefore plays a prominent role in regulating cell fate.Exploring biomaterial-cell interactions is an effective way to elucidate ECM-cell interactions and to engineer materials mimicking cell natural environment. The chemical composition of biomaterials surfaces can alter the conformation of adsorbed proteins which subsequently affect cell response. We are interested in investigating how biomaterial surfaces with well-defined chemistries elicit specific responses of human mesenchymal stem cells (hMSCs) such as adhesion, differentiation and migration.

To this respect, copolymers with controlled ratios of PMA and PEA were used: 100/0, 70/30, 50/50, 30/70, 0/100. Surfaces and FN adsorption were characterised by AFM imaging and immunostaining. ELISAs were also carried out to characterise the availability of binding domains of FN and the adsorption of BMP-2. Preliminary cell experiments using hMSCs were conducted as well in order to investigate initial cell attachment, cell adhesion and osteogenic differentiation.

Regarding surface characterisation, results demonstrated that FN is organises into a network-like formation on PEA. The network is disrupted with increase concentration of PMA on which it forms globular aggregates. ELISAs revealed that important binding areas of FN are exposed differently on the surfaces. However, higher adsorption of BMP-2 was found on PEA. Preliminary cell experiments indicate that hMSCsadhere on the surfaces via the formation of focal adhesions.

This work aims at deciphering how surface-dependent FN organisation can influence cell-biomaterial interactions in general and stem cell differentiation in particular. A graded organisation of FN is
obtained upon adsorption on p(MA-co-EA) copolymers. The family of surfaces promote cell attachment and focal adhesion formation, and
might trigger different cell response in terms of, e.g, differentiation and migration.
Summary: Biomaterials aim to mimic the properties of the cell native environment in order to direct specific cell responses . We are interested in exploring the differentiation potential of human mesenchymal stem cells (hMSCs) using chemically similar polymers with controlled ratios of PEA and PMA. Cell-biomaterial interactions are mediated via a layer of fibronectin (FN), a major component of the ECM, adsorbed onto the P(EA-co-MA) surfaces. References: Salmerón-Sánchez , M.; Ricoa, P.; Moratal, D.; Lee, T. T.; Schwarzbauer, E. J.; Garcia, J. A., Role of material-driven fibronectin fibrillogenesis in cell differentiation. Biomaterials 2011; Vol. 32.Report abuse »
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