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Graphene oxide modulates the steroidogenic and apoptotic pathway in a buffalo granulosa cell model
EP30980
Poster Title: Graphene oxide modulates the steroidogenic and apoptotic pathway in a buffalo granulosa cell model
Submitted on 07 Nov 2019
Author(s): E.L. Lyngdoh, Varij Nayan, M. Vashisht, S. Kumari, T. Bhatia, Jasmer, S. Pawaria, A. Bhardwaj, P. Kumar, P. Sikka, S.K. Onteru, D. Singh
Affiliations: 1. ICAR-Central Institute for Research on Buffaloes, Hisar-125001, Haryana, India; 2. CAR-National Dairy Research Institute, Karnal-132001, Haryana, India; 3. ICAR-National Research Centre on Equines, Hisar-125001, Haryana, India
This poster was presented at Golden Jubilee International Conference on "New Millennia Agriculture- Novel Trends and Future Scenario" (November 6-8, 2019) at CCS Haryana Agricultural University, Hisar-125001, Haryana, India
Poster Views: 85
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Poster Information
Abstract: Background: Endocrine Disrupting Chemicals (EDCs) are exogenous substances or mixture that alter functions of the endocrine system, causes adverse health effects in an intact organism, its progeny or (sub) populations (WHO, 2002). The dioxins, bisphenol A, persistent organic pollutants (POP), pesticides are among those EDCs. Effects of EDCs cross entire lifespan, with disease burden & costs of US$127 billion annually in Europe & $340 billion in USA (Attina et al., 2016). The engineered nanomaterials (ENMs) are chemicals or materials that are engineered. For example, they can be AuNP, AgNP, Graphene Oxide (GO) among several others. Recent progress in the field of nanotechnology has led to increased exposure to ENMs by animals & humans. However, little is known about the effects of ENMs on reproductive functions. Recent research on ENMs has indicated that they have the potential to perturb the Hypothalamus-Pituitary-Gonadal axis. Graphene Oxide (GO) is one of the ENMs and widely being used and may exert nanotoxicity through ROS. Therefore, we explored the effects of graphene oxide on the steroidogenic and apoptotic pathway in buffalo granulosa cells.

Objectives: The goals of this study are: (1) to understand the effects of GO on genes associated with steroidogenesis in a buffalo granulosa cell model; and (2) to understand the effects of GO on genes associated with apoptotic pathway in a buffalo granulosa cell model; and (3) to understand the effects of GO on exerting oxidative stress and modulation of progesterone hormone in buffalo granulosa cell model

Methodology: 1. In vitro culture of buffalo ovary Granulosa cells; 2. Characterization of graphene oxide nanomaterial; 3. Co-incubation of buffalo granulosa cells with graphene oxide and assessing the effects: Granulosa cells were incubated with 2 different concentrations of graphene oxide for 24 h.

Results: 1. Graphene oxide modulates steroidogenesis in the buffalo granulosa cell model. 2. Graphene oxide affects the apoptotic pathway in the buffalo granulosa cell model. 3. Graphene oxide affects the progesterone hormone levels during buffalo granulosa cell culture. 4. Graphene oxide does not exert oxidative stress through lipid peroxidation & anti-oxidant induction in the buffalo granulosa cell model.

Discussion & Conclusion: GO were characterized by TEM, HRTEM, SAED, DLS, etc. GO modulates steroidogenesis in the buffalo granulosa cell model by affecting the gene expression of β-Hsd and Cyp19A1. GO affects the apoptotic pathway in the buffalo granulosa cell model by affecting the gene expression of Bax and caspase-3. Significant changes in progesterone levels were found during granulosa cell culture on GO supplementation. The GO is not exerting oxidative stress through anti-oxidant induction & lipid Peroxidation in buffalo granulosa cell model. The ENMs such as GO may modulate the endocrine system and have the potential to affect the steroidogenic pathway.

Future Perspectives: The rapid growth in Nanotechnology and Engineered Nanomaterials (ENMs) calls into questions about their endocrine modulating abilities, health implications, bioethical and regulatory issues.

Acknowledgments: ICAR-CIRB, ICAR-NDRI, ICAR-NRCE; Funding: IXX12688
Summary: We explored the effects of graphene oxide on the steroidogenic and apoptotic pathway in buffalo granulosa cells. (Best Poster Award)References: (1) WHO, 2002;
(2) Attina et al., 2016, Lancet Diabetes Endocrinol.
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