« Back
High speed untargeted 4D-lipidomics LC-MS/MS workflows with Parallel Accumulation Serial Fragmentation (PASEF)
Poster Title: High speed untargeted 4D-lipidomics LC-MS/MS workflows with Parallel Accumulation Serial Fragmentation (PASEF)
Submitted on 24 Jul 2019
Author(s): Ulrike Schweiger-Hufnagel1, Aiko Barsch1, Sven W. Meyer1
Affiliations: 1Bruker Daltonik GmbH, 28359 Bremen, Germany
Poster Views: 1,257
View poster »

Poster Information
Abstract: The search for new and validated biomarkers is of particular interest in clinical areas like oncology1,2 or neurology3. As lipids play an important role in many diseases, the area of lipidomics has become central for clinical research. While there is a more in-depth oriented approach to ID as many lipids as possible, clinically-oriented projects often demand a high-throughput for large sample cohorts. Therefore, a short cycle time per sample is necessary to realize research projects with hundreds or even thousands of samples in a reasonable time frame. In order to keep up with this, the analytical instrumentation needs to deliver a high data quality at high acquisition speeds. This is realized by the PASEF (Parallel Accumulation Serial Frag-mentation) acquisition mode on the timsTOF Pro system.4Summary: The potential of the PASEF acquisition mode to increase the sample throughput was demonstrated. The crucial ability to separate co-eluting isobaric compounds and to identify differences between sample groups was maintained. With this, PASEF is demonstrated to be an optimal acquisition mode for deep profiling applying longer LC gradient times as well as for projects with high turnover needs, e.g. in clinical metabolomics studies. References: (1) Röhrig, F., Schulze, A., Nat. Rev. Cancer 16, 732–749 (2016)
(2) Vriens, K. et al., Nature 566, 403–406 (2019)
(3) Yang, Q., Vijayakumar, A. & Kahn, B. B., Nat. Rev. Mol. Cell Biol. 19, 654–672 (2018)
(4) Meier, F. et al., J. Proteome Res. 14, 5378–5387 (2015)
(5) Shevchenko, A. et al., J. Lipid Res., 49, 1137-1146 (2008)
(7) Bowden, J. A. et al., J. Lipid Res. 58, 2275–2288 (2017)
Report abuse »
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters

The Impact of Lithocholic Acid as A Surfactant on the Characteristics and Cytocompatibility of Azithromycin Loaded Self-Emulsifying Drug Delivery System
Reem Abou Assi1,2, Ibrahim M.Abdulbaqi1,2, Toh Seok Ming1, Chan Siok Yee1*, Habibah A. Wahab1*, Yusrida Darwis1*

Immunity Buster Herbal Plant: During Covid-19 Pandemic
Saurav Suryavanshi

Cosmetics Quality and Safety Testing Technology
Tylor Keller

CCS-centric protein identification using IM Score
Robin Park1, Patrick Garrett3, Sven Brehmer2, Titus Jung3, Vijayaraja Gnanasambandan1, Marc-Antoine Beauvais1, Sebastian Wehner2, Lennard Pfennig2, Hyunsoo Kim3, Christopher Adams1, Dennis Trede2, John R. Yates, III3, Rohan Thakur1 1Bruker Daltonics Inc., USA 2Bruker Daltonik GmbH, Bremen, Germany 3Scripps Research, San Diego, USA

Real-time search of Ubiquitin diGLY modified peptides and PaSER acquisition control on the timsTOF PRO
Christopher Adams1; Robin Park1; Nagarjuna Nagaraj4; Sven Brehmer4; Matt Willetts3; Elizabeth Gordon3; Barry M. Zee2; Hayley Peckham2; Kimberly Lee2; Tharan Srikumar3 1Bruker,San Jose,CA;2Cell Signaling Technologies, Danvers, MA;3Bruker, Billerica, MA; 4Bruker, Bremen, Germany