« Back
High-throughput detection of Glycine in biological samples based on a novel enzymatic assay
Poster Title: High-throughput detection of Glycine in biological samples based on a novel enzymatic assay
Submitted on 15 Mar 2018
Author(s): Gustavo Chavarria, Ph.D., Kyle Schmitt, Ph.D., Nidhi Tibrewal, Ph.D., Sonika Saddar, Ph.D., Preeti Saini, Ph.D., Grigoriy Tchaga, Ph.D. and Gordon Yan, Ph.D.
Affiliations: BioVision Inc.
Poster Views: 753
View poster »

Poster Information
Abstract: Glycine, is a neutral, non-essential, amino acid. It is the only proteinogenic amino acid
without a chiral center. However, it has been established that Glycine is an important
neurotransmitter, being found in the central nervous system in specific locations such
as the spinal and brainstem. Glycine utilizes rapid postsynaptic inhibition that is
necessary for hearing processing, and pain transmission processes. Glycine is also a
potent glutamate coagonist on NMDA receptors. Abnormal concentrations are found in
patients suffering hyperglycinemia, and schizophrenia. Standard procedures for
quantitation of glycine involve NMR, MS, and HPLC and immunoassay techniques.
However, these protocols are not ideal for high-throughput screening of large number
of samples or compound screening. Here, we report a simple, convenient and highthroughput
enzymatic assay using an improved Glycine Oxidase (GO) Isozyme and a
fluorophore in a 96-well format. Our results demonstrate that glycine serum in control
vs patient samples are comparable to those published in literature. The assay provides
scientist the ability to analyze large number of samples and provides a novel tool in the
field of neuroscience research.
Summary: • BioVision’s Glycine Assay Kit is a high-throughput screening assay for measuring Glycine concentration
in different biological samples using an enzyme that specifically recognizes this amino acid and a
• Glycine Assay Kit provides a simple, safe, sensitive and reproducible method of measuring Glycine.
• Numerous factors that affect Glycine metabolism can be studied using this kit.
Report abuse »
Creative Commons

Related Posters

The Optimum Food for AIDS Patients’ Health Support
Mhamd salih imad hamdi under the supervision of Dr reem Abu Assi

Streamlining Biologics Development with the Expi Expression Systems
Chao Yan Liu, Jian Liu, Wanhua Yan, Kyle Williston, Kenneth Thompson, Maya Yovcheva, Sara Barnes, Mark Bundy, Melissa Cross, Katy Irvin, Joaquín Canay, Mary Reynolds, Natasha Lucki, Henry Chiou, Andrew Campbell, Jonathan Zmuda

Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A

Primary Human Hepatocytes 3D in vitro Culture Model for Studying Hepatic Function
Sujoy Lahiri, Julia Tritapoe, Kate Comstock, Michael F. Millett, Mark Kennedy, Theresa V. Nguyen, Deborah K. Tieberg, David T. Kuninger

Guillermo Salamanca Grosso; Monica Patricia Osorio Tangarife; David Garcia Mendez; Julien Wist