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High-throughput detection of Glycine in biological samples based on a novel enzymatic assay
EP27368
Poster Title: High-throughput detection of Glycine in biological samples based on a novel enzymatic assay
Submitted on 15 Mar 2018
Author(s): Gustavo Chavarria, Ph.D., Kyle Schmitt, Ph.D., Nidhi Tibrewal, Ph.D., Sonika Saddar, Ph.D., Preeti Saini, Ph.D., Grigoriy Tchaga, Ph.D. and Gordon Yan, Ph.D.
Affiliations: BioVision Inc.
Poster Views: 687
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Poster Information
Abstract: Glycine, is a neutral, non-essential, amino acid. It is the only proteinogenic amino acid
without a chiral center. However, it has been established that Glycine is an important
neurotransmitter, being found in the central nervous system in specific locations such
as the spinal and brainstem. Glycine utilizes rapid postsynaptic inhibition that is
necessary for hearing processing, and pain transmission processes. Glycine is also a
potent glutamate coagonist on NMDA receptors. Abnormal concentrations are found in
patients suffering hyperglycinemia, and schizophrenia. Standard procedures for
quantitation of glycine involve NMR, MS, and HPLC and immunoassay techniques.
However, these protocols are not ideal for high-throughput screening of large number
of samples or compound screening. Here, we report a simple, convenient and highthroughput
enzymatic assay using an improved Glycine Oxidase (GO) Isozyme and a
fluorophore in a 96-well format. Our results demonstrate that glycine serum in control
vs patient samples are comparable to those published in literature. The assay provides
scientist the ability to analyze large number of samples and provides a novel tool in the
field of neuroscience research.
Summary: • BioVision’s Glycine Assay Kit is a high-throughput screening assay for measuring Glycine concentration
in different biological samples using an enzyme that specifically recognizes this amino acid and a
fluorophore
• Glycine Assay Kit provides a simple, safe, sensitive and reproducible method of measuring Glycine.
• Numerous factors that affect Glycine metabolism can be studied using this kit.
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