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High Throughput Screening for Host Cell Proteins with sub ppm sensitivity using dia-PASEF with a 15 minute gradient
EP38448
Poster Title: High Throughput Screening for Host Cell Proteins with sub ppm sensitivity using dia-PASEF with a 15 minute gradient
Submitted on 09 Mar 2022
Author(s): Stuart Pengelley1, Eckhard Belau1, Waltraud Evers1, Christina Bell1, Michael Greig2, Detlev Suckau1
Affiliations: 1Bruker Daltonics GmbH & Co. KG, Bremen, Germany 2Bruker Scientific LLC, San Jose, USA
Poster Views: 197
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Poster Information
Abstract: Host Cell Proteins (HCPs) usually remain in biopharmaceutical drug preparations such as monoclonal antibodies and need to be removed as much as possible using a combination of purification steps. Mass spectrometry has emerged as a method for identifying and monitoring HCPs throughout the manufacturing pipeline. Here, we apply parallel accumulation – serial fragmentation combined with data-independent acquisition (dia-PASEF(1)) in a high throughput approach, demonstrating HCP detection and quantitation with sub ppm sensitivity.Summary: Here, we apply parallel accumulation – serial fragmentation combined with data-independent acquisition (dia-PASEF(1)) in a high throughput approach, demonstrating HCP detection and quantitation with sub ppm sensitivity.References: 1. Meier et al., Mol. Cell. Proteom. 2018, 17: 2534-2545
2. VIP-HESI dual source brochure (Bruker)
3. Meier et al., Nat Methods. 2020 Dec ;17(12) :1229-1236
4. Huang et al., Anal. Chem 2017, 89, 5436-5444
5. Molden et al., Mabs 2021, Jan-Dec;13(1):1955432
6. Venn Diagram created using Venny 2.1: Oliveros J.C.
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