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Identification of microRNA targets using microRNA modulation techniques and gene expression arrays
EP22797
Poster Title: Identification of microRNA targets using microRNA modulation techniques and gene expression arrays
Submitted on 10 Mar 2015
Author(s): Emily M. Anderson, Maren Mayer, Kevin Sullivan, Barbara Robertson, Žaklina Strezoska, Annaleen Vermeulen, and Devin Leake
Affiliations: GE Healthcare Dharmacon, Inc.
Poster Views: 2,585
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Poster Information
Abstract: MicroRNAs (miRNAs) have been shown to regulate gene expression through both translational attenuation and degradation of messenger RNAs (mRNAs). Though these small noncoding RNAs are predicted to play a signicant role in development, differentiation, and disease etiology, validation of miRNA targets remains a challenge. miRNA mimics and inhibitors are valuable tools for elucidating the roles of miRNAs. Here we have transfected a human liver cell line alternately with a miRNA mimic and inhibitor to miR-122 and analyzed mRNA expression changes by whole genome microarray. By examining the overlap of messages down-regulated by miRNA mimics and up-regulated by miRNA inhibitors, we robustly identify miRNA-regulated messages, many of which have canonical seed matches and some which are not identied by standard target prediction programs.Summary: By examining the overlap of messages down-regulated by miRNA mimics and up-regulated by miRNA inhibitors, we robustly identify miRNA-regulated messages, many of which have canonical seed matches and some which are not identied by standard target prediction programs.References: D. Baek, J. Villén, et al. The impact of microRNAs on protein output. Nature. 455(7209), 64-71 (2008).

M. Beitzinger, L. Peters, et al. Identification of human microRNA targets from isolated argonaute protein complexes. RNA Biol. 4(2), 76-84 (2007).

G. Easow, A.A. Teleman, et al. Isolation of microRNA targets by miRNP immunopurification. RNA. 13(8), 1198-11204 (2007).

F.V. Karginov, C. Conaco, et al. A biochemical approach to identifying microRNA targets. Proc. Natl. Acad. Sci. U S A. 104(49), 19291-19296 (2007).

L.P. Lim, N.C. Lau, et al. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature. 433(7027), 769-773 (2005).

F.E. Nicolas, H. Pais, et al. Experimental identification of microRNA-140 targets by silencing and overexpression miR-140. RNA. 14(12), 1-8 (2008).

M. Selbach, B. Schwanhäusser, et al. Widespread changes in protein synthesis induced by microRNAs. Nature. 455(7209), 58-63 (20
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