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Identification of new alternative-lengthening-of-telomeres-positive cancer cell lines using the C-Circle assay.
Poster Title: Identification of new alternative-lengthening-of-telomeres-positive cancer cell lines using the C-Circle assay.
Submitted on 09 Jun 2015
Author(s): Andrei G. Malykh1, Jonathan R. Doyle1, Fang Tian2, Joyce HY Lee3, Jeremy D. Henson4, Roger R. Reddel3.
Affiliations: 1Capital Biosciences, Inc., Gaithersburg, MD; 2ATCC, Manassas, VA; 3Children's Medical Research Institute, Sydney, Australia; 4UNSW Australia, Sydney, Australia
This poster was presented at AACR 2015 Annual Meeting
Poster Views: 2,753
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Poster Information
Abstract: Alternative lengthening of telomeres (ALT) is a telomere maintenance mechanism used by approximately 10% of human cancers, including poor prognosis childhood and adult cancers for which development of better treatments is a priority. Currently there are only about ten published ALT+ cancer cell lines available for research, only four of which are available from international repositories. The development of the C-Circle Assay has made it possible to accurately and conveniently test a large number of cell lines for ALT activity. We have tested a panel of 297 cancer cell lines from the American Type Culture Collection (ATCC) with the C-Circle Assay for ALT and identified 34 new ALT+ cell lines. Importantly, we have identified for the first time ALT+ cancer cell lines derived from breast carcinoma, hepatocellular carcinoma, melanoma and leukemia. The overall proportion of ALT+ cancer cell lines was 11.4% (n = 297), which included 19.5% of leukemia (n = 46), 19.0 % of melanoma (n= 21), 12.7% of non-small cell lung carcinoma (n = 53) and 3.3% of breast carcinoma (n = 37) cell lines. These results identify additional cancer cell line resources for research into the ALT mechanism, including cell lines from cancer types where ALT has not previously been studied in detail.
This study was partially supported by the NIH SBIR Grant 1R43CA168124-01A1 to CBI.
Summary: New ALT[+] cell lines in ATCC collection have been identified.References: 1. Reddel RR, Bryan TM, Colgin LM, Perrem KT, Yeager TR. Alternative lengthening of telomeres in human cells. Radiat.Res. 2001 Jan;155(1 Pt 2):194-200.
2. Henson JD, Hannay JA, McCarthy SW, Royds JA, Yeager TR, Robinson RA, et al. A robust assay for alternative lengthening of telomeres in tumors shows the significance of alternative lengthening of telomeres in sarcomas and astrocytomas. Clin.Cancer Res. 2005 Jan 1;11(1):217-225.
3. Shay JW, Wright WE. The reactivation of telomerase activity in cancer progression. Trends Genet. 1996 Apr;12(4):129-131.
4. Holt SE, Wright WE, Shay JW. Regulation of telomerase activity in immortal cell lines. Mol.Cell.Biol. 1996 Jun;16(6):2932-2939.
5. Lau LSM et al. Detection of alternative lengthening of telomeres by telomere quantitative PCR. Nucleic Acids Res. 2013 January; 41(2): e34. Published online 2012 August 24. doi: 10.1093/nar/gks781. PMCID: PMC3553966
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