Posters
« Back
Identification of novel double-stranded RNAs responsible for regulation of antibiotic resistant genes in the agricultural environment
EP25799
Poster Title: Identification of novel double-stranded RNAs responsible for regulation of antibiotic resistant genes in the agricultural environment
Submitted on 04 Jun 2017
Author(s): Dorota Dobrzanska, Lauren Acton, Jess Rollason, Igor Morozov
Affiliations: School of Life Sciences, James Starley Building, Coventry University, CV1 5FB, Coventry, UK
This poster was presented at Gordon Research Coneference
Poster Views: 857
View poster »


Poster Information
Abstract: The discovery and development of first antibiotic in 1920’s led to significant improvement of effective therapies for human health. Unfortunately, together with increased number of new antibiotics, the number of pathogenic bacteria resistant to antibiotics have gradually increased. There are two main mechanisms of acquiring antibiotic resistance: mutation or horizontal gene transfer (HGT). Current research demonstrated that quinolone (qnr) and polymixin (mcr-1) resistance genes are present on plasmids originated from aqua and agricultural communities non-related to clinical environment, suggesting that the origin of antibiotic resistance comes from natural environments. The mechanism of action of antibiotic is well studied, however the understanding of expression of antibiotic resistant genes on level of non-coding RNAs still remains unknown. The metatranscriptomics analyses of RNAs in microbial communities revealed significant amount of double stranded RNAs that do not match any known DNA databases. This study would lead to understanding of dynamic shifts in gut microbiota (particularly in dsRNAs) and highlight the key regulatory pathways and novel structural genes responsible for microbial adaptation to antibiotic treatment. Results from this research would be directly relevant to RNA virology by discovery of uncharacterised dsRNA in bacterial communities. It will shift current knowledge about antibiotic resistance and allow prediction of regulatory pathways of AR resistant genes. Results from this research have significant potential for pharmaceutical industry in developing novel strategies and designing new generation therapies (antisense RNAs or novel RNA-based drugs) to target antibiotic resistant bacteria. The results of this research are also relevant to a wide microbial research community to initiate studies on the regulation of expression of novel genes and non-coding element in the development and transmission of resistance within the human host.Summary: Results from this research would be directly relevant to RNA virology by discovery of uncharacterised dsRNA in bacterial communities. It will shift current knowledge about antibiotic resistance and allow prediction of regulatory pathways of AR resistant genes. Results from this research have significant potential for pharmaceutical industry in developing novel strategies and designing new generation therapies (antisense RNAs or novel RNA-based drugs) to target antibiotic resistant bacteria.References: AHMED, Z. 2016. Antibiotic Resistance: A Real Threat to Life. Annals Abbasi Shaheed Hospital & Karachi Medical & Dental College, 21, 250-251.
BAKER, C. L., et al. 2016. Design of a Real-Time Multiplex PCR Assay for Select Antibiotic Resistance Genes. Journal of Molecular Diagnostics, 18, 971-971.
CHAUDHARY, A. S. et al. 2016. A review of global initiatives to fight antibiotic resistance and recent antibiotics' discovery. Acta Pharmaceutica Sinica B, 6, 552-556.
LALAK, A., 2016. et al. Mechanisms of cephalosporin resistance in indicator Escherichia coli isolated from food animals. Veterinary Microbiology, 194, 69-73.
LE DEVENDEC, L., et al. 2016. Impact of colistin sulfate treatment of broilers on the presence of resistant bacteria and resistance genes in stored or composted manure. Veterinary Microbiology, 194, 98-106.
NAGOBA, B. S., et al. 2016. Norfloxacin Induced Cross-Resistance to Fluoroquinolones and Non fluoroquinolone Groups of Antimicrobial Agents in Pseudo
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Multiplex miRNA Profiling for Biomarker Discovery and Verification Studies Using the FirePlex® Platform
M. Tackett, B. Heinrich, I. Diwan, G. Tejada, C. Rafferty, E. Atabakhsh, and D. Pregibon

PEAKS™ Software for the processing of timsTOF Pro PASEF data: identification and label-free quantitation
Markus Lubeck(1), Heiner Koch(1), Scarlet Beck(1), Paul Savage(2), Oliver Raether(1), Pierre-Olivier Schmit(2) Paul Shan (3) and Gary Kruppa (4)

Platinum™ SuperFi™ DNA Polymerase for the highest success in PCR
Rasa Sukackaitė, Martyna Simutytė, Skaistė Valinskytė, Laurynas Vanagas, Karolis Matjošaitis, Renata Rimšelienė, Remigijus Skirgaila.

Plasma proteomic profiling one year postpartum of women with Pre-eclampsia shows dysregulated cardiometabolic profile
Fatma S. Abad, Antigoni Manousopoulou, Diana J. Garay-Baquero, Brian R. Birch, Bas B. van Rijn, Spiros D. Garbis and Bashir A. Lwaleed

DNA-free Platinum Taq DNA polymerase for reliable microbiome studies
Kęstutis Bargaila, Vytautas Budrys, Andrius Krasauskas, Dovilė Lisauskienė, Milda Romeikaitė, Sonata Jurėnaitė-Urbanavičienė,Ramunė Leipuvienė and Juozas Šiurkus