Posters
« Back
IGF1 Biomarker Assay Validation
EP20215
Poster Title: IGF1 Biomarker Assay Validation
Submitted on 19 Dec 2013
Author(s): Michael Robinson, Urban A. Kiernan, Dobrin Nedelkov, Kemmons A. Tubbs, Eric E. Niederkofler, Randall W. Nelson
Affiliations: Intrinsic bioprobes
Poster Views: 842
View poster »


Poster Information
Abstract: As proteomic initiatives make headway in biomarker discovery andgeneral protein cataloguing, it becomes imperative to be able to repeatedly screen for the same target proteins from thousands ofindividuals for clinical investigation and biomarker validation. Clincal proteomics technologies have matured over the pastdecade, conventional approaches are still not capable of routine lyperforming such analyses and have therefore been subjugated by classical immunoassay platforms for such population screening.

However, these methodologies are inherently blind to the structural diversity found within any human population that are caused by genetic and/or post translational modifications, which may ultimately influence biomarker behavior. Such problems can be resolved through novel affinity-based mass spectrometry approaches. Shown here is the development and validation of such a mass spectrometric immunoassay (MSIA), targeting the human biomarker insulin like growth factor 1. 
Summary: This work demonstrates the development and validation of a novel clinical proteomics approach in which specific protein targets can be expeditiously and selectively isolated from acomplex biological fluid for MS characterization.Report abuse »
Creative Commons

Related Posters


TMT Quantification for Shotgun Proteomics on a Trapped-Ion-Mobility quadrupole-Time-Of- Flight mass spectrometer (TIMS-QTOF) powered by Parallel-Accumulation and Serial- Fragmentation (PASEF)
Matt Willetts1, Shourjo Ghose1 Heiner Koch2, Scarlet Koch2, Markus Lubeck2, Gary Kruppa1

BAT Molecular Imaging with SPECT-CT, PET-CT, PET-MRI and Fluorescence-PET: A Systematic Review of the Literature Data
Tarik Z Belhocine, MD., Ph.D *, Albert A Driedger, MD., Ph.D **, Jean-Luc Urbain, MD., Ph.D ***

PEAKS™ Software for the processing of timsTOF Pro PASEF data: identification and label-free quantitation
Markus Lubeck(1), Heiner Koch(1), Scarlet Beck(1), Paul Savage(2), Oliver Raether(1), Pierre-Olivier Schmit(2) Paul Shan (3) and Gary Kruppa (4)

Plasma proteomic profiling one year postpartum of women with Pre-eclampsia shows dysregulated cardiometabolic profile
Fatma S. Abad, Antigoni Manousopoulou, Diana J. Garay-Baquero, Brian R. Birch, Bas B. van Rijn, Spiros D. Garbis and Bashir A. Lwaleed

The Good, the Bad and the Ugly: Selective single cell isolation
Sandra Lubos1,2, Nils Körber1, Heide Marie Resch1, Iris Augustin2, Stefan Niehren1