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Improved Cellular Maintenance of Human Pluripotent Stem Cells in a Serum-Free, Xeno-Free Culture System for Clinical Applications
EP38434
Poster Title: Improved Cellular Maintenance of Human Pluripotent Stem Cells in a Serum-Free, Xeno-Free Culture System for Clinical Applications
Submitted on 09 Mar 2022
Author(s): Deborah L.D. Maybruch, PhD¹, Philip D. Manos², Brad Hamilton³, Michelle M. Janaszak¹, Tanya Potcova¹
Affiliations: ¹Biological Industries USA, Cromwell, Connecticut | ²EverCell Bio, Inc., Natick, Massachusetts | ³Lifevault Bio, Waltham, Massachusetts
This poster was presented at ISSCR 2020
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Poster Information
Abstract: The potential of human pluripotent stem cells (hPSCs) continues to show incredible promise, rapidly progressing through
clinical trials for use in the treatment of many diseases. However, historical methods of hPSC culture have remained a
barrier of entry into the clinic even with relative success within in vitro cellular research. While traditional protocols often
require the use of animal-derived feeder layers and supplements, a defined and carefully formulated microenvironment for
hPSC culture becomes increasingly critical for entry to the clinic. Ultimately, for the therapeutic application of clinically
relevant cells to be more standardized, media formulations and culture conditions must be refined and optimized while
fully supporting proper phenotype and genetic stability over long-term culture.
Currently, the recommendation for transitioning hPSC research to the clinic is to maintain cells in xeno-free culture conditions.
While determining the ideal xeno-free environment remains challenging for technical and biological reasons, there are multiple commercially available formulations for hPSC maintenance that are either conventional and serum-free with animal-derived components, or completely xeno-free. Each available media has varying levels of growth factor supplementation intended to maintain pluripotency and prevent differentiation, but not all formulations are created
equally and thus may cause variation in pluripotency potential and stability.
This study examines the fundamental variations in morphology and pluripotency of hPSCs in commercially available culture systems to better understand the effects of media formulations on hPSC characteristics. Findings demonstrate that the xeno-free, serum-free NutriStem® hPSC Medium yields the highest levels of pluripotency and retains viability over long-term cultures. Furthermore, transitions from each media into this supportive environment are explored to validate
efficient methods of obtaining high-performing, clinically compliant hPSCs for therapeutic applications.
Summary: This study examines variations in morphology and pluripotency of hPSCs in commercially available culture systems. Findings demonstrate that the xeno-free, serum-free NutriStem® hPSC Medium yields the highest levels of pluripotency and retains viability over long-term cultures. Furthermore, transitions from each media into this supportive environment are explored to validate efficient methods of obtaining high-performing, clinically compliant hPSCs for therapeutic applications.References: Report abuse »
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