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EP29526
Poster Title: Improved Expansion of Neural Stem Cells with Gibco™ Heat Stable Recombinant Human Basic Fibroblast Growth Factor
Submitted on 03 Jan 2019
Author(s): Brittany Balhouse, Diana Navarro, Richard Josephson, and Matthew Dallas
Affiliations: Thermo Fisher Scientific
This poster was presented at 2018 ASCB|EMBO Annual Meeting
Poster Views: 437
Submitted on 03 Jan 2019
Author(s): Brittany Balhouse, Diana Navarro, Richard Josephson, and Matthew Dallas
Affiliations: Thermo Fisher Scientific
This poster was presented at 2018 ASCB|EMBO Annual Meeting
Poster Views: 437
Abstract: Basic fibroblast growth factor (bFGF) is used in neural stem cell (NSC) media to maintain multipotency. Native bFGF rapidly loses biological activity when exposed to culture conditions (37°C), Heat Stable (HS) bFGF maintains > 90% homology to the native protein and ≥ 80% biological activity, even after 72 hours of exposure to 37°C. In human embryonic stem cell-derived NSCs, HS bFGF can maintain multipotency and standard doubling times with reduced bFGF concentrations. In primary rat NSCs, using HS bFGF allows for a more user-friendly workflow without the loss of multipotency or slower proliferation. After expansion, HS bFGF can be removed just as easily as native bFGF to allow for downstream differentiation into neurons and glial cells.Summary: HS bFGF: Engineered for greater stabilityReferences:
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