We've updated our Privacy Policy to make it clearer how we use your personal data.
We use cookies to provide you with a better experience, read our Cookie Policy
EP29526
Poster Title: Improved Expansion of Neural Stem Cells with Gibco™ Heat Stable Recombinant Human Basic Fibroblast Growth Factor
Submitted on 03 Jan 2019
Author(s): Brittany Balhouse, Diana Navarro, Richard Josephson, and Matthew Dallas
Affiliations: Thermo Fisher Scientific
This poster was presented at 2018 ASCB|EMBO Annual Meeting
Poster Views: 896
Submitted on 03 Jan 2019
Author(s): Brittany Balhouse, Diana Navarro, Richard Josephson, and Matthew Dallas
Affiliations: Thermo Fisher Scientific
This poster was presented at 2018 ASCB|EMBO Annual Meeting
Poster Views: 896
Abstract: Basic fibroblast growth factor (bFGF) is used in neural stem cell (NSC) media to maintain multipotency. Native bFGF rapidly loses biological activity when exposed to culture conditions (37°C), Heat Stable (HS) bFGF maintains > 90% homology to the native protein and ≥ 80% biological activity, even after 72 hours of exposure to 37°C. In human embryonic stem cell-derived NSCs, HS bFGF can maintain multipotency and standard doubling times with reduced bFGF concentrations. In primary rat NSCs, using HS bFGF allows for a more user-friendly workflow without the loss of multipotency or slower proliferation. After expansion, HS bFGF can be removed just as easily as native bFGF to allow for downstream differentiation into neurons and glial cells.Summary: HS bFGF: Engineered for greater stabilityReferences:
Ask the author a question about this poster.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Related Posters
Is There an Association Between H. pylori infection and MALT lymphoma development ?!
ALAA AHMED ALI SLEEM
Kinase Stable Cell Lines
Creative Biogene
Stable-Cell-Line
Creative Biogene
Cytotoxicity of Environmental Toxins PFOA and Gen X
Lauren Zane, Thomas Schultz
ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
Kenneth Thompson, Maya Yovcheva, Sara Barnes, Melissa Cross, Katy Irvin, Natasha Lucki, Henry Chiou, and Jonathan Zmuda