Posters
« Back
Improved Expansion of Neural Stem Cells with Gibco™ Heat Stable Recombinant Human Basic Fibroblast Growth Factor
EP29526
Poster Title: Improved Expansion of Neural Stem Cells with Gibco™ Heat Stable Recombinant Human Basic Fibroblast Growth Factor
Submitted on 03 Jan 2019
Author(s): Brittany Balhouse, Diana Navarro, Richard Josephson, and Matthew Dallas
Affiliations: Thermo Fisher Scientific
This poster was presented at 2018 ASCB|EMBO Annual Meeting
Poster Views: 671
View poster »


Poster Information
Abstract: Basic fibroblast growth factor (bFGF) is used in neural stem cell (NSC) media to maintain multipotency. Native bFGF rapidly loses biological activity when exposed to culture conditions (37°C), Heat Stable (HS) bFGF maintains > 90% homology to the native protein and ≥ 80% biological activity, even after 72 hours of exposure to 37°C. In human embryonic stem cell-derived NSCs, HS bFGF can maintain multipotency and standard doubling times with reduced bFGF concentrations. In primary rat NSCs, using HS bFGF allows for a more user-friendly workflow without the loss of multipotency or slower proliferation. After expansion, HS bFGF can be removed just as easily as native bFGF to allow for downstream differentiation into neurons and glial cells.Summary: HS bFGF: Engineered for greater stabilityReferences: Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Reward Modulates Local Field Potentials, Spiking Activity and Spike-Field Coherence in the Primary Motor Cortex
Junmo An, Taruna Yadav, John P Hessburg, Joseph T Francis

THERAPEUTIC EFFICACY OF OPEN VERSUS CLOSED KINETIC STRENTH TRAINING WITH BILATERAL AFO FOR THE TREATMENT OF SPASTICITY DIPLEGICS GAIT CONDITIONS -COMPARATIVE STUDY
Karthikeyan T

Accelerated Ageing in Depression: A Study of Two Cohorts
Mathew A. Harris, Laura de Nooij, Xueyi Shen, Toni-Kim Clarke, Riccardo Marioni, Simon R. Cox, Emma L. Hawkins, Mark J. Adams, Liana Romaniuk, Stephen M. Lawrie, James H. Cole, Andrew M. McIntosh and Heather C. Whalley

Genetic Engineering in Male Sterility for Hybrid Variety Development
Abir Hasan Joy

VITVO: Mimicking In Vivo Complexity By The Innovative 3D Model
Olivia Candini1, Giulia Grisendi1, Elisabetta Manuela Foppiani1, Matteo Brogli1, Beatrice Aramini2, Valentina Masciale3, Carlotta Spano1, Tiziana Petrachi4, Elena Veronesi4, Pierfranco Conte5,6, Giorgio Mari1 & Massimo Dominici1,3