Posters
« Back
Improved sensitivity and higher lipid annotation ID capabilities using a new vacuum insulated heated ESI source
EP38443
Poster Title: Improved sensitivity and higher lipid annotation ID capabilities using a new vacuum insulated heated ESI source
Submitted on 09 Mar 2022
Author(s): Matthias Szesny, Lucy Woods, Sven Meyer, Ansgar Korf, Nikolas Kessler, Aiko Barsch
Affiliations: Bruker Daltonics GmbH & CoKG, 28359 Bremen, Germany
Poster Views: 119
View poster »


Poster Information
Abstract: Abundance of lipids in typical samples such as plasma extracts can vary drastically. Therefore, analytical equipment with a high sensitivity and dynamic range is required in lipidomics research. Here, we present results of a new vacuum insulated heated ion source (VIP-HESI) coupled with a timsTOF Pro ion mobility mass spectrometer including trapped ion mobility separation and CCS values.
As part of the evaluation process dilution series of a lipid standard with and without matrix were acquired to assess the limit of detection (LOD) and the dynamic range. In a second experiment, the lipid ID annotation capability was examined using a commercially available serum standard.
All experiments were compared to data which was acquired with the standard Bruker Apollo II source.
Summary: Here, we present results of a new vacuum insulated heated ion source (VIP-HESI) coupled with a timsTOF Pro ion mobility mass spectrometer including trapped ion mobility separation and CCS values. Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


4D Lipidomics workflow utilizing PASEF acquisition mode for increased throughput
Florian Zubeil 1 , Ansgar Korf 1 , Viola Jeck 1 , Aiko Barsch 1 , Nikolas Kessler 1 , Sven W. Meyer 1

Non-targeted metabolomics ID workflows providing custom annotation confidence reporting: CCS-enabled annotation workflows
Aiko Barsch1; Matthias Szesny1; Ulrike Schweiger-Hufnagel1; Sofie Weinkouff1, Nikolas Kessler1

Metabolomics Core Facility provides ready to use data exploration with MetaboScape
Jörg Büscher 1 , Sofie Weinkouff 2 , Ansgar Korf 2 , Aiko Barsch 2 , Nikolas Kessler 2

Lipidomics profiling of plasma samples from patients with SARS CoV 2 by timsTOF Pro 2 PASEF
Jun Yang 1 , Aaron Peng 1 , Xuejun Peng 2 , Lucy Woods 2

Enhancing metabolic phenotyping by increased sensitivity provided by a vacuum insulated probe heated ESI (VIP-HESI) source
Melvin Gay1; Aude-Claire Morillon2; Sven Myers3; Magdalene Reinkensmeier3; Aiko Barsch3; Nicola Gray2; Berin Boughton2; Nathan Lawler2; Jeremy Nicholson2,4; Luke Whiley2