« Back
Improved T Cell Activation Bioassays for Development of Bispecific Antibodies and Engineered T Cell Immunotherapies
Improved T Cell Activation Bioassays for Development of Bispecific Antibodies and Engineered T Cell Immunotherapies
Submitted on 15 May 2017

Pete Stecha, Denise Garvin, Jim Hartnett, Frank Fan, Mei Cong and Zhi-jie Jey Cheng
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
Poster Views: 1,261
View poster »
Poster Abstract
Immunotherapy aims to boost a patient’s own immune system to fight disease. In recent years, a variety of immunotherapy strategies aimed at inducing, strengthening or engineering T cell responses have emerged as promising approaches for the treatment of cancer and autoimmune disease.

Here we describe a platform of T cell activation bioassays for the development of CD3 bispecific antibodies and engineered T cell immunotherapies.

Specifically, we developed two bioluminescent reporter-based bioassays to measure T cell activation via CD3 (NFAT-RE) or CD3 + CD28 (IL-2 promoter). These

Corresponding author: richard.somberg@promega.comReport abuse »
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters

Extracorporeal Shock Wave Therapy in situ – a novel approach to obtain an activated fat graft
Priglinger Eleni1,2, Peterbauer Anja2,3, Wurzer Christoph1,2,4, Steffenhagen Carolin1,2, Redl Heinz1,2,4, Sandhofer Matthias5, Wolbank Susanne 1,2

Artificial multimeric proteins with programmable biological and chemical functions
Piotr M. Skowron, Łukasz Janus, Olga Żołnierkiewicz, Małgorzata Skowron, Joanna Jeżewska-Frąckowiak, Daria Krefft, Dawid Nidzworski, Kasjan Szemiako, Natalia Maciejewska, Marta Nowak, Alicja Wiercińska-Drapało, Grzegorz Węgrzyn, Aneta Szymańska, Agnieszka Żylicz-Stachula

A quick potency assay for osteogenic and chondrogenic differentiation and evaluation of donor variability of adipose derived stem cells
Eleni Oberbauer1,3, Susanne Wolbank1,3, Carolin Steffenhagen1,3, Ara Hacobian1,3, Georg Feichtinger1,3, Christian Gabriel2,3, Heinz Redl1,3, Florian Hildner2,3

Development of an Automated Platform Assay for Potency Measurement of Drug Substances
Veerendra Koppolu

Mechanisms of vasculogenesis in 3D fibrin matrices mediated by the interaction of adipose-derived stem cells and endothelial cells
Sabrina Rohringer1,2, Severin Mühleder1,2, Pablo Hofbauer1,2, Heinz Redl1,2, Susanne Wolbank1,2, Wolfgang Holnthoner1,2