Posters
« Back
Intact Protein LC-MS: How to Overcome the Challenges?
EP21993
Poster Title: Intact Protein LC-MS: How to Overcome the Challenges?
Submitted on 10 Jun 2014
Author(s): Robert van Ling, Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, Mauro DePra, Christian Ravnsborg, Dafydd Milton, Remco Swart
Affiliations: Thermo Fisher Scientific
Poster Views: 2,068
View poster »


Poster Information
Abstract: Mass-spectrometry-based protein characterization finds two major applications in top-down proteomics and biopharma analysis of recombinant proteins, antibodies and other biotherapeutics. However, analysis of high-molecular-weight proteins can be challenging. The methodology chosen should minimize common problems in protein analysis such as degradation, poor recovery, broad elution profiles, poor ionization efficiency, ion sensitivity, and resolution. To address these performance challenges, we compared different prototype column-spray interfaces.Summary: Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


PERFIL DE COMPONENTES VOLATILES DE MIELES FLORALES Y MONOFLORALES COLOMBIANAS
Salamanca,G. G. , Osorio, T. M.; Scriche, R. I.

NEW INSULAR RED PROPOLIS FROM COLOMBIA: BOTANICAL ORIGIN, BIOLOGICAL AND CHEMICAL MARKERS
Salamanca Grosso, G.; Osorio Tangafarife, M.P.

Unique Users-Initiated Biotransformation Reactions for Analysis of Xenobiotic Metabolism
Richard Lee, Rytis Kubilius, Vitaly Lashin, Alexandr Sakharov, Anne Marie Smith

Automated Deformulation of LC/MS and GC/MS Data through Database Searching
Anne Marie Smith, Richard Lee, Artsiom Piatrouski, Andrey Paramonov, Vitaly Lashin

Screening of Common Spices Against Multi-Drug Resistant Mycobacterium tuberculosis
Swati Singh1, Arpita Batta1,2,  Maria Kulsoom1, T. N. Dhole2, B. N. Mishra1, Vineeta Singh1