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Intact Protein LC-MS: How to Overcome the Challenges?
EP21993
Poster Title: Intact Protein LC-MS: How to Overcome the Challenges?
Submitted on 10 Jun 2014
Author(s): Robert van Ling, Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, Mauro DePra, Christian Ravnsborg, Dafydd Milton, Remco Swart
Affiliations: Thermo Fisher Scientific
Poster Views: 2,327
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Poster Information
Abstract: Mass-spectrometry-based protein characterization finds two major applications in top-down proteomics and biopharma analysis of recombinant proteins, antibodies and other biotherapeutics. However, analysis of high-molecular-weight proteins can be challenging. The methodology chosen should minimize common problems in protein analysis such as degradation, poor recovery, broad elution profiles, poor ionization efficiency, ion sensitivity, and resolution. To address these performance challenges, we compared different prototype column-spray interfaces.Summary: Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.
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