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Integrated platform including automated bligh and dyer extraction and dual-column
Poster Title: Integrated platform including automated bligh and dyer extraction and dual-column
Submitted on 18 Jun 2014
Author(s): Emmanuel Varesio, Guenter Boehm, Sandra Jahn, Renzo Picenoni, Gérard Hopfgartner
Affiliations: Life Sciences Mass Spectrometry, University of Geneva, Switzerland; CTC Analytics AG, Zwingen, Switzerland
This poster was presented at ASMS 2014
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Abstract: Sample preparation workflows for metabolomic studies of tissues or cells require most of the time a Bligh and Dyer extraction or one of its variant (e.g. the Folch extraction). This step is cumbersome and generally performed manually in order to separate the aqueous fraction containing polar endogenous metabolites from the
organic fraction containing apolar compounds like lipids. Proteins remain at the interface of the two solvents.
Here we propose to integrate an automated Bligh and Dyer extraction on a robotic system including a dual-column UHPLC-MS/MS platform for the metabolomic analysis of tissues or cells. The aqueous fraction is split and analysed sequentially at two different mobile phase pH values, whereas the lipidic fraction is analysed alternately with an extended gradient.
Summary: • Automated Bligh and Dyer extraction for metabolomic studies.
• Dual-column UHPLC setup for the analysis of the polar and lipidic fractions.
• Alternating acidic and basic mobile phase for the separation of the polar fraction.
• Identification of unknown compounds by SWATH HR MS2 spectra acquisition.
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