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Investigating the Effects of Commercial Antimicrobial Agents on Human Corneal Epithelial Cell Membranes
EP22852
Poster Title: Investigating the Effects of Commercial Antimicrobial Agents on Human Corneal Epithelial Cell Membranes
Submitted on 27 Mar 2015
Author(s): Ian J. Horner, Jerod J. Hurst, Nadine D. Kraut, Alyssa A. Rook, Crystal M. Collado, G Ekin-Atilla Gokcumen, and Frank V. Bright
Affiliations: SUNY University at Buffalo
This poster was presented at Pittcon
Poster Views: 1,947
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Poster Information
Abstract: Multi-purpose solutions (MPS) are a single solution that functions to simultaneously rinse, disinfect, clean and store soft contact lenses. Several commercial MPS products contain polyhexamethylene biguanide (PHMB) and/or polyquaternium-1 (PQ-1) as antimicrobial agents. In this proster we report the effects of PHMB and PQ-1 on small unilamellar vesicles (SUV) that we have designed to mimic the average human corneal epithelial cell membrane. Specifically, we assessed the interactions of PHMB and PQ-1 on the biomembrane by using fluorescence spectroscopy, dynamic light scattering (DLS), and liquid chromatography/mass spectrometry (LC-MS). Fluorescence assessed the membrane surface polarity and stability through the temperature-dependent generalized polarization (GP), the gel-to-liquid transition temperature (Tm) and the associated van’t Hoff enthalpy (ΔHVH) as a function of PHMB and PQ-1 concentration. DLS evaluated SUV aggregation as a function of PHMB and PQ-1 concentration and SUV composition. LC-MS determined the composition of any precipitates that formed. PHMB association with the mimic SUV bilayer leads to: (i) a decrease in surface polarity, (ii) an increase in (Tm), (iii) an increase in phospholipid-phospholipid cooperativity, and (iv) an increase in SUV size on a nanometer scale. PQ-1 association with the mimic SUV bilayer leads to: (i) an increase in surface polarity (ii) no change in (Tm), (iii) no change in phospholipid-phospholipid cooperativity, and (iv) an increase in SUV size on a micron scale due to SUV aggregate formation. The aggregates exhibited a phospholipid composition equivalent to the SUV prior to the addition of PQ-1. These results are consistent with PHMB adsorbing onto and PQ-1 intercalating into the mimic SUV bilayer structures.
Summary: Several commercial multi-purpose solutions (MPS) products contain polyhexamethylene biguanide (PHMB) and/or polyquaternium-1 (PQ-1) as antimicrobial agents. In this poster we report the effects of PHMB and PQ-1 on small unilamellar vesicles (SUV) that we have designed to mimic the average human corneal epithelial cell membrane. References: Missel, P. J.; Lang, J. C.; Rodeheaver, D. P.; Jani, R.; Chowhan, M. A.; Chastain, J.; Dagnon, T., Drugs Pharm. Sci. 2009, 189, 101-189.
Willcox, M. D.; Phillips, B.; Ozkan, J.; Jalbert, I.; Meagher, L.; Gengenbach, T.; Holden, B.; Papas, E., Optom. Vis. Sci. 2010, 87, 839-46.
Gilbert, P.; Moore, L. E., J. Appl. Microbiol. 2005, 99, 703-15.
Bright, F. V.; Merchea, M. M.; Kraut, N. D.; Maziarz, E. P.; Liu, X. M.; Awasthi, A. K., Cornea 2012, 31, 1480-8.
Merchant, T. E.; Lass, J. H.; Roat, M. I.; Skelnik, D. L.; Glonek, T., Curr. Eye Res. 1990, 9, 1167-76.
Parasassi, T.; De, S. G.; d'Ubaldo, A.; Gratton, E., Biophys J 1990, 57, 1179-86.
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