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 Isolation and Characterization of Host Mutations that Suppress the Bacteriophage Lambda Rex Phenotype
EP25227
Poster Title: Isolation and Characterization of Host Mutations that Suppress the Bacteriophage Lambda Rex Phenotype
Submitted on 17 Jan 2017
Author(s): Hibah Alattas, Roderick Slavcev
Affiliations: University of Waterloo -Pharmacy School
This poster was presented at Bacteriophage 2017
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Poster Information
Abstract: The Bacteriophage lambda (λ) T4rII exclusion (Rex) phenotype is defined as the inability of T4rII mutant bacteriophage to form plaques on a lawn of E. coli lysogenized by bacteriophage λ. More than six decades since the discovery of Rex by Seymour Benzer in 1955, yet the mechanism behind this elusive exclusion system remains a mystery. The Rex system is encoded by two genes of λ (rexA and rexB). The onset of the Rex phenotype, somehow triggered by T4rII infection of a Rex+ lysogen, results in rapid membrane depolarization and a harsh cellular environment that in many ways resembles the stationary phase in metabolism and morphology. Despite some cell killing, infected lysogens can to some extent recover from Rex activation. The phenotype may thus be a mutualistic protection mechanism that protects both itself and the host cell from external super-infection. In this study, I have designed a system for the rapid one-step isolation of host mutations that attenuate this phenotype, in order to identify the host genes involved in Rex and elucidate the mechanism of this enigmatic exclusion system. I was able to isolate some host mutations of E. coli K-12 that abrogate the Rex phenotype while simultaneously identifying for the first time several outer membrane protein genes including: ompA, ompF, ompW, and ompX that are essential in imparting Rex activity. In analyzing these omp mutants it was noted that several of these identified omp candidates play a critical role in cellular osmotic balance and the onset of stationary phase. This work substantiates a model whereby the onset of Rex shunts cells into a temporary stationary phase-like state that inhibits T4rII phage growth. Summary: Isolation and Characterization of Host Mutations that Suppress the Bacteriophage Lambda Rex Phenotype
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