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Label-free quantification of oxidized peptides in eHAP cell lines via a high-throughput dia-PASEF workflow
EP38464
Poster Title: Label-free quantification of oxidized peptides in eHAP cell lines via a high-throughput dia-PASEF workflow
Submitted on 09 Mar 2022
Author(s): Romano Hebeler 1, Renata Blatnik 1, Philipp Strohmidel 1, Hamish Pegg 2, Joanna Kirkpatrick 2
Affiliations: 1 Bruker Daltonics GmbH & Co. KG, Bremen, Germany 2 The Francis Crick Institute, London, United Kingdom
Poster Views: 343
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Poster Information
Abstract: dia-PASEF® (Meier et al., 2020; 1) merges the benefits of data-independent acquisition (DIA) with the advantages of ion mobility in proteomics experiments (Meier et al., 2018; 2). The ion mobility dimension improves the alignment of precursor and fragment spectra. In this study, we applied a dia-PASEF workflow in combination with an Evosep One (Evosep, Bache et al., 2018, 3) chromatographic system for high-throughput analysis of eHAP cell line digests, while quantifying spiked-in, oxidized target peptides in SpectronautTM (Biognosys, Bruderer et al., 2015, 4) on different gradient lengths.Summary: In this study, we applied a dia-PASEF workflow in combination with an Evosep One (Evosep, Bache et al., 2018, 3) chromatographic system for high-throughput analysis of eHAP cell line digests, while quantifying spiked-in, oxidized target peptides in SpectronautTM (Biognosys, Bruderer et al., 2015, 4) on different gradient lengths.References: 1: https://pubmed.ncbi.nlm.nih.gov/33257825/
2: https://www.biorxiv.org/content/10.1101/336743v1.full
3: https://www.mcponline.org/content/17/11/2284
4: https://pubmed.ncbi.nlm.nih.gov/25724911/
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