Posters
« Back
LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction for the Analysis of Polar and Non-polar Metabolites in Biological Samples
EP25962
Poster Title: LC-SWATH/MS Metabolomics Platform with Hyphenation of Extraction for the Analysis of Polar and Non-polar Metabolites in Biological Samples
Submitted on 28 Jun 2017
Author(s): Michel Raetz 1, Renzo Picenoni 2, Guenter Boehm 2, Gérard Hopfgartner 1
Affiliations: 1 Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva, Switzerland; 2 CTC Analytics, Zwingen, Switzerland
This poster was presented at HPLC 2017, Prague
Poster Views: 2,037
View poster »


Poster Information
Abstract: Metabolomics studies are still challenging and technically demanding. Both sample preparation and data acquisition remain the bottlenecks in the measurements. On one hand, due to the large
chemical space of the analytes and their broad dynamic range that need to be dealt with. On the other hand, metabolomics still suffer from limited selectivity due to the structural similarities
within the low molecular weight compounds.
SWATH/MS opened new possibilities within Data Independent Aquisition (DIA) to gain selectivity for -omics studies as all fragments form all precursors in different isolation windows are recorded.
The hyphenated setup allows full automation from sample preparation over chromatography to MS data acquisition and feature annotation. The integrated dual LC configuration with three different
mobile phases enables a broad coverage of the metabolome. Additionally, the online SPE based lipid fractionation allows to equalize the dynamic range of the abundance of lipid classes.
Summary: • Fully automated robotic sample preparation with hyphenated dual LC-SWATH/MS analysis for lipids and polar metabolites
• Automated SPE based lipid fractionation to equalize lipid abundances and reduce of runtimes
• SWATH/MS acquisition to record all the product ions originating from specific precursor ion windows
in order to measrue lipids and polar metabolites
• Automated library search for polar metabolites using an in-house accurate mass MS/MS library
References: Y. Zhang et al. The Use of Variable Q1 Isolation Windows Improves Selectivity in LC–SWATH–MS Acquisition. J. Proteome Res., 2015.

T. Bruderer et al. In silico prediction for the investigation of comedication interferences in quantitative LC–MS detection in the SRM mode. Bioanalysis. 2012.

R. Bonner et al. SWATH acquisition mode for drug metabolism and metabolomics investigations. Bioanalysis, 2016.
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Multiplex miRNA Profiling for Biomarker Discovery and Verification Studies Using the FirePlex® Platform
M. Tackett, B. Heinrich, I. Diwan, G. Tejada, C. Rafferty, E. Atabakhsh, and D. Pregibon

PEAKS™ Software for the processing of timsTOF Pro PASEF data: identification and label-free quantitation
Markus Lubeck(1), Heiner Koch(1), Scarlet Beck(1), Paul Savage(2), Oliver Raether(1), Pierre-Olivier Schmit(2) Paul Shan (3) and Gary Kruppa (4)

PERFIL DE COMPONENTES VOLATILES DE MIELES FLORALES Y MONOFLORALES COLOMBIANAS
Salamanca,G. G. , Osorio, T. M.; Scriche, R. I.

MALDI Imaging Mass Spectrometry for the study of cardiovascular pathology
Takashi Nirasawa1, Megumi Terada2,3, Hiroko Namba2, Nobuto Kakuda2, Patrick Bruneval3, Hatsue Ishibashi-Ueda4 and Masaya Ikegawa1

Distinct deposition of amyloid-bspecies in brains with Alzheimer’s disease pathology visualized with MALDI imaging mass spectrometry
Nobuto Kakuda1, Tomohiro Miyasaka1, Takashi Nirasawa2, Shigeo Murayama3, Yasuo Ihara1 and Masaya Ikegawa1