Posters
« Back
LC/MS/MS Quantitation of Eicosapentaenoic Acid in Rat Plasma
EP21826
Poster Title: LC/MS/MS Quantitation of Eicosapentaenoic Acid in Rat Plasma
Submitted on 10 Apr 2014
Author(s): Rachel Sun, Jordan Nally, Tim Shoaf
Affiliations: BASi
Poster Views: 1,020
View poster »


Poster Information
Abstract: Since EPA appears in many different forms, it will need to be released from conjugated forms by hydrolysis in order to quantitate total EPA in plasma samples. Plasma samples were first incubated under acidic conditions (ACN/6N HCl) for 1 hour at 100C to release EPA, liquid-liquid extraction with hexanne followed to clean up the samples from the matrix. EPA-d5 was added as an internal standard before hydrolysis. Hexand supernatant was evaporated to dryness, reconstittued with ACN/water and injected into an LC/MS/MS using a Kinetex XB-C18 column with an ammonium acetate/ACN/H20 mobile phase. TSQ quantum instrument at negative mode was used for detection/quantitation.Summary: Eicosapentaenoic acid (EPA) is an omega-3 fatty acid, which has been documented to be important in biochemical and physiological processes including inflammatory response, blood clotting and the immune system. Eicosapentaenoic acid (EPA) appears in different forms in the blood including triglyceride, ethyl ester, or free acid. In this work we presented an LC/MS/MS method to quantitate the total eicosapentaenoic acid in rat plasma with a 96-well plate format.Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Degradation of Artificially Aged Vegetable-Tanned Leather using RP-HPLC and FTIR-ATR
Yadi Hu, Eleni Tziamourani, SC Boyatzis, Jingru Wang, Lvyang Wang, Keyong Tang

The Optimum Food for AIDS Patients’ Health Support
Abu_bakir Imad hamdi

2D FT0ICR MS/MS analysis of IgG1
Johanna Paris 1 , Tomos E. Morgan 1 , Yuko P. Y. Lam 1 Christopher A. Wootton 1 Mark André Delsuc 2 , Mark P. Barrow 1 , John O’Hara 3 , and Peter B. O’Connor 1

High speed untargeted 4D-lipidomics LC-MS/MS workflows with Parallel Accumulation Serial Fragmentation (PASEF)
Ulrike Schweiger-Hufnagel1, Aiko Barsch1, Sven W. Meyer1

TMT Quantification for Shotgun Proteomics on a Trapped-Ion-Mobility quadrupole-Time-Of- Flight mass spectrometer (TIMS-QTOF) powered by Parallel-Accumulation and Serial- Fragmentation (PASEF)
Matt Willetts1, Shourjo Ghose1 Heiner Koch2, Scarlet Koch2, Markus Lubeck2, Gary Kruppa1