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Localisation of HN Gene Expression of NDV-AF2240 in Brain of 4T1 Induced Breast Cancer BALB/c mice
Poster Title: Localisation of HN Gene Expression of NDV-AF2240 in Brain of 4T1 Induced Breast Cancer BALB/c mice
Submitted on 29 Nov 2018
Author(s): Umar Ahmad, Gholamreza Motalleb, Asmah Rahmat, Fauziah Othman, & Aini Ideris
Affiliations: Department of Human Anatomy, Faculty of Medicine & Health Sciences, University Putra Malaysia (UPM), Serdang, Selangor, Malaysia; Unit of Neuroscience, Department of Anatomy, Faculty of Medical Sciences, Bauchi State University, Gadau, Nigeria.; Department of Biology, Faculty of Science, University of Zabol, Zabol, Iran.; Department of Nutrition, Faculty of Medicine & Health Sciences, University Putra Malaysia (UPM), Serdang, Selangor, Malaysia; Department of Virology, Faculty of Veterinary Medicine, University Putra Malaysia (UPM), Serdang, Selangor, Malaysia.
This poster was presented at Asian Pacific Society of Neurochemistry (APSN)
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Poster Information
Abstract: Newcastle disease virus (NDV) known as avian paramyxovirus type-1 (APMV1) belongs to the genus Avulavirus in a family Paramyxoviridae. NDV is shown to have promising oncolytic properties. Our overall goal in this study is to localise the presence of HN gene expression of oncolytic NDV-AF2240 in the brain of 4T1 induced breast cancer BALB/c mice using molecular techniques. A total of 300 female BALB/c mice were divided randomly into 15 groups (5 non cancerous groups, 10 cancerous groups) consisting of 20 mice per group. The mice in cancerous groups were inoculated sub-cutaneously with 4T1 cells; co-cultured either with NDV AF2240 or/and tamoxifen for four weeks. Localisation of HN gene expression of NDV-AF2240 in the brain tissue were performed using in situ RT-PCR, immunolabelling and confocal laser scanning microscopy (CLSM) and negative staining and transmission electron microscopy (NSTEM). There was no significant (p>0.05) different in the brain weight between cancer control (CC) and all the cancerous groups. HN gene expression was detected in both the brain and its blood vessels. No significant different (p>0.05) in HN gene intensity between CT/NDV8 and CT/NDV16 groups, however, it was significantly different (p<0.05) compared to CT/NDV32 and CT/NDV64 groups. CLSM and NSTEM results revealed the presence of NDV-AF2240 in the brain tissues of cancerous groups, but not in the normal groups treated with virus. These findings showed that NDV-AF2240 could be use to treat any types of brain tumour due to its oncoselectivity to transformed malignant brain cells while leave the non transformed brain cells unharmed.
Summary: Newcastle disease virus (NDV) is being developed for cancer treatment and is selectively infecting and killing cancer cells. However, it circulation to other part of the body is likely to cause some minimal sides effect especially in the brain, hence, the need to localised its presence in the brain by identifying the key players in NDV mediated oncolysis particularly the HN gene expression in a Brain of 4T1 Induced Breast Cancer BALB/c mice..References: Alexander, D. J., Manvell, R. J., Lowings, J. P., Frost, K. M., Collins, M. S., Russell, P. H., and Smith, J. E. (1997). Avian Pathology: Journal of the W.V.P.A 26, 399–418.
Scheid, A., and Choppin, P. W. (1973). Journal of Virology 11, 263–271.
Tan, W. S., C. H. Lau, B. K. Ng, A. L. Ibrahim and K. Yusoff (1995). DNA sequence 6, 47-50. Zamarin, D., and Palese, P. (2012). Future Microbiol 7(3), 347-367.
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