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Lyo-ready SuperScript Reverse Transcriptases: High-Performance Lyophilization-Compatible Enzymes for RT-PCR-based assays
EP29305
Lyo-ready SuperScript Reverse Transcriptases: High-Performance Lyophilization-Compatible Enzymes for RT-PCR-based assays
Submitted on 16 Oct 2018

Inga Šerėnaitė, Darius Kavaliauskas, Monika Raižytė, Viktorija Urbonė, Gitana Timofejevaitė, Andrejus Naumovas, Juozas Šiurkus. Thermo Fisher Scientific Baltics UAB, V. A. Graičiūno 8, Vilnius, Lithuania

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Poster Abstract
Nucleic acid detection assays in lyophilized (freezedried) format provide many advantages over conventional wet format in terms of higher stability and lower shipping costs. Conventional formulations of reverse transcriptases (RTs) used for RT-PCR contain up to 50% of glycerol, which serves as a cryoprotectant and stabilizes enzymes for extended periods of time. However, even at such minute concentrations as 0.5%, glycerol can interfere with the lyophilization process. Therefore, most of the conventional enzyme preparations are incompatible with lyophilization.

We would like to thank Eglė Merkienė, Jurgita Rubekina, Dovilė Litvinavičiūtė.
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