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Maximizing cell wash performance with the AquaMax® 2000/4000 microplate washers
EP20228
Poster Title: Maximizing cell wash performance with the AquaMax® 2000/4000 microplate washers
Submitted on 19 Dec 2013
Author(s): Cathy Olsen, Iris Yang, and James Wasson
Affiliations: Molecular Devices
Poster Views: 824
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Poster Information
Abstract: Many cell-based applications, including calcium flux assays, cell-based ELISAs, ion channel assays, and others require gentle but thorough washing of cells in order to ensure cell retention, cell integrity and optimal assay performance. The 96 cell wash head for AquaMax® 2000/4000 microplate washers from Molecular Devices uses angled pins to dispense fluid without disturbing cells. Adjustable dispense rate and aspiration height allow users to minimize disruption of even weakly adherent cells. We demonstrate 95% retention of weakly adherent HEK293 cells on a standard tissue culture microplate surface after washing with the AquaMax microplate washer and cell wash head. We also show excellent performance of washed CHO-M1 cells in a Fluo-4 calcium flux assay run on the FlexStation® 3 multimode microplate reader.
Summary: Many cell-based applications, including calcium flux assays, cell-based ELISAs, ion channel assays, and others require gentle but thorough washing of cells in order to ensure cell retention, cell integrity and optimal assay performance. The 96 cell wash head for AquaMax® 2000/4000 microplate washers from Molecular Devices uses angled pins to dispense fluid without disturbing cells.
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