Posters
« Back
Measuring the 2nd Virial Coeffient and Molecular Density of Proteins to Improve Crystallization
EP22751
Poster Title: Measuring the 2nd Virial Coeffient and Molecular Density of Proteins to Improve Crystallization
Submitted on 02 Mar 2015
Author(s): Michel Terray, John Stenson
Affiliations: Malvern Instruments Ltd
This poster was presented at GT Bio, Grenoble, France
Poster Views: 1,494
View poster »


Poster Information
Abstract: One of the challenges in biopharmaceutical development is measuring the 3-d structure of the active protein. The diffraction of X-ray beams through a crystalline structure can be measured to produce a 3-d picture of the density of electrons within a sample. From this the position of atoms in that sample as well as their chemical bonds can be determined. X-ray crystallography is able to help identify the structure and therefore the function of the active protein. A key issue with this technique is the necessity for the sample to be in a crystalline form. With proteins the crystallisation process can be difficult, as solvent conditions have a significant impact on the ability of a sample to form crystals.

The second virial coefficient (B22 or A2) is a thermodynamic parameter which describes the interaction strength between a protein and a solvent, and can be correlated with protein solubility. This parameter is used as an indicator of the potential for a protein to crystallise within a given solvent. Negative virial coefficient values are indicative of attractive interactions, with macromolecules tending to self-associate. Positive virial coefficient values are indicative of repulsive interactions, with the macromolecules preferring solvation.

It is possible to use Static Light Scattering (SLS) detectors to measure the second virial coefficient of a protein. This parameter can be measured either using a batch SLS detector or by a Size Exclusion Chromatography (SEC) linked SLS detector.

In this poster a Malvern Instruments SEC-MALS 20 detector was connected to a Refractive Index (RI) concentration detector and an online differential viscometer (DP). This has the advantage of not only measuring the protein molecular weight, and subsequently the second virial coefficient, but also the Intrinsic Viscosity (IV) of a sample. The IV is inversely proportional to the molecular density of a sample so can be used to provide information about the samples conformation as well as allowing the hydrodynamic radius (Rh) to be calculated.

The second virial coefficient of Human Serum Albumin (HSA) has been measured using two different buffer conditions, Phosphate buffer pH 7.4 and Citrate buffer pH 4.1. Interesting and contrasting data was obtained for these two buffer conditions
Summary: The second virial coefficient of Human Serum Albumin (HSA) was measured using a SEC-MALS 20 detector connected to a Refractive Index concentration detector and an online differential viscometer. Two different buffer conditions, Phosphate buffer pH 7.4 and Citrate buffer pH 4.1 were used, which revealed interesting and contrasting dataReport abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


2D FT0ICR MS/MS analysis of IgG1
Johanna Paris 1 , Tomos E. Morgan 1 , Yuko P. Y. Lam 1 Christopher A. Wootton 1 Mark André Delsuc 2 , Mark P. Barrow 1 , John O’Hara 3 , and Peter B. O’Connor 1

Diagnostic outcomes of MRE as an investigation for NSS of small bowel disease: Can CTE be a cost-effective alternative in patients above 50 years old?
K ElGendy, E Musgrave, M Twemlow

Appropriateness of MRCP use in a DGH
A Elawad, B Billimoria

An audit on compliance with the Ottawa Knee Rule in patients with traumatic knee pain
Qasim Afzaal, Saad SIddiqui, Amit Shah

Segmental
Kashtwari D*, Alamoudi. A