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Mechanisms of vasculogenesis in 3D fibrin matrices mediated by the interaction of adipose-derived stem cells and endothelial cells
Mechanisms of vasculogenesis in 3D fibrin matrices mediated by the interaction of adipose-derived stem cells and endothelial cells
Submitted on 06 Oct 2017

Sabrina Rohringer1,2, Severin Mühleder1,2, Pablo Hofbauer1,2, Heinz Redl1,2, Susanne Wolbank1,2, Wolfgang Holnthoner1,2
1Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Centre, Vienna, Austria 2Austrian Cluster for Tissue Regeneration, Vienna, Austria
This poster was presented at EUSAAT 2015, Linz, Austria
Poster Views: 367
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Poster Abstract
Vascularization of tissue-engineered constructs is essential to provide sufficient nutrient
supply and hemostasis after implantation into target sites. Co-cultures of adipose-derived stem cells
(ASC) with outgrowth endothelial cells (OEC) in fibrin gels were shown to provide an effective
possibility to induce vasculogenesis in vitro. However, the mechanisms of the interaction between
these two cell types remain unclear so far. The aim of this study was to evaluate differences of direct
and indirect stimulation of ASC-induced vasculogenesis, the influence of ASC on network stabilization
and molecular mechanisms involved in vascular structure formation. Endothelial cells (EC) were
embedded in fibrin gels either containing non-coated or ASC-coated microcarrier beads as well as
ASC alone. Moreover, EC-seeded constructs incubated with ASC-conditioned medium were used in
addition to constructs with ASC seeded on top. Vascular network formation was visualized by green
fluorescent protein (GFP) expressing cells or immunostaining for CD31 and quantified. RT-qPCR of
cells derived from co-cultures in fibrin was performed to evaluate changes in the expression of EC
marker genes during the first week of culture. Moreover, angiogenesis-related protein levels were
measured by performing angiogenesis proteome profiler arrays. The results demonstrate that proximity
of endothelial cells and ASC is required for network formation and ASC stabilize EC networks by
developing pericyte characteristics. We further showed that ASC induce controlled vessel growth by
secreting pro-angiogenic and regulatory proteins. This study reveals angiogenic protein profiles
involved in EC/ASC interactions in fibrin matrices

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