Posters
« Back
METAVIRAL EVIDENCE FOR PHAGES TRANSDUCTING ANTIBIOTICS RESISTANCE GENES IN DIFFERENT ENVIRONMENTS
EP25204
Poster Title: METAVIRAL EVIDENCE FOR PHAGES TRANSDUCTING ANTIBIOTICS RESISTANCE GENES IN DIFFERENT ENVIRONMENTS
Submitted on 02 Jan 2017
Author(s): Starikova E., Kovarsky B.
Affiliations: Scientific Research Institute of Physical-Chemical Medicine, Moscow
Poster Views: 1,182
View poster »


Poster Information
Abstract: Later increases in antibiotics resistance are linked to broad antibiotics usage in medicine and industry and to therefore changing evolutionary processes in microbial communities. Mobile genetic elements such as plasmids, transposones and bacteriophages play a certain role in antibiotics resistance expansion.
The objective of our study was to look for evidence for potential bacteriophage-mediated antibiotics resistance (AR) gene transfer through in silico analysis of metagenomic VLP sequences. In order to do that, we compared 257 publicly availiable metavirome sequences to antibiotics resistance genes sequences from CARD database.
As a result, we detected various types of AR genes in 21 (8%) of the analyzed viromes in human gut, coral reef, peatlands and saltern inlet environments. 33% of the AR genes discovered were beta-lactamase genes. The obtained results can be considered as evidence for phage-mediated antibiotics resistance genes transfer.
Summary: This research is an in silico meta-analysis of the publicly availiable metaviromes. Our objective of our study was to look for potential evidence for bacteriophage-mediated antibiotics gene transfer events.References: 1. Healthy human gut phageome – 2016 – Manrique et al., PNAS, 113(37):10400–5
2. Disease-specific alterations in the enteric virome in inflammatory bowel disease – 2015 – Norman et al., Cell, 160(3):447-60
3. Study of the viral and microbial communities associated with Crohn's disease: a metagenomic approach – 2013 – Pérez-Brocal et al., Clin Transl Gastroenterol
4. Viruses in a 14th-Century Coprolite – 2014 – Appelt et al., Appl Environ Microbiol, 80(9): 2648–55.
5. The Pacific Ocean Virome (POV): A Marine Viral Metagenomic Dataset and Associated Protein Clusters for Quantitative Viral Ecology – 2013 – Hurwitz et al., PLoS ONE, 8(2)
6. Viral and microbial community dynamics in four aquatic environments-- 2013 – Rodriguez-Brito et al., ISME J., 4(6):739-51
7. Combining genomic sequencing methods to explore viral diversity and reveal potential virus-host interactions – 2015 – Cheryl-Emiliane et al., Front. Microbiol, 6:265
8. Assessing the Diversity and Spec
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons