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MICRONUCLEI AS BIOMARKERS OF GENETIC DAMAGE IN ORAL SQUAMOUS CELL CARCINOMA
EP31311
Poster Title: MICRONUCLEI AS BIOMARKERS OF GENETIC DAMAGE IN ORAL SQUAMOUS CELL CARCINOMA
Submitted on 14 Feb 2020
Author(s): Nancy A. Shaaban 1 BDS, Hanaa S. Raslan 2 PhD, Omneya M. Ramadan 3 PhD, Ahmed S. Habib 4 PhD, Eiman I. Zaki 5 PhD
Affiliations: 1. Instructor of Oral Pathology, Faculty of Dentistry, Alexandria University. 2. Professor of Oral Pathology. 3. Lecturer of Oral Pathology. 4. Assistant Professor of Cranio Maxillofacial and Plastic Surgery. 5. Lecturer of Histology and Cell Biology, Faculty of Medicine, Alexandria University
This poster was presented at 2nd Annual Oral Pathology Scientific Day. Faculty of Dentistry, Cairo University
Poster Views: 189
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Poster Information
Abstract: Introduction:
Oral cancer is a major health problem, causing high morbidity and mortality rates. Oral Squamous Cell Carcinoma (OSCC) accounts for 90-95% of all oral malignancies. The prognosis of OSCC is often poor due to the late discovery of most lesions, after they have reached a large size. Hence the importance of early diagnosis of cancer which raises the five year survival rate to 90% as opposed to 20% in case of late diagnosis. Here comes the role of biomarkers of genetic damage that can have excellent utility in early diagnosis of cancer. Many investigators have already called micronuclei (MN) an upcoming biomarker of tumorogenesis as it is considered an objective, non-invasive method for early detection of genetic damage. More than 90% of human malignancies originate from epithelial cells. Thus the MN test in exfoliated buccal epithelial cells could be used as a tool for biomonitoring the genetic damage in high risk human populations and for screening cellular alteration in OSCC cases.
Aim of the study: To assess the degree of genetic damage in the oral squamous cell carcinoma lesions using micronuclei as biomarkers.
Materials and Method: A total of thirty four participants; seventeen OSCC patients and17 healthy control subjects were included. Cytological smears were taken from the lesion of the OSCC cases as well as from the buccal mucosa of the control group subjects using a cytobrush. Cytological smears were stained using Papanicolaou stain and the number of micronucleated (MNed) cells per 1000 cells was determined for each subject.
Results: There was a statistically significant difference between the number of MNed cells in the cytological smears of OSCC cases and those of the healthy control subjects.
Conclusion: The number of MNed cells increases significantly in cancer patients thus they can be considered as an important biomarker for genetic damage.
Summary: Micronuclei (MN) are useful as biomarkers of tumorogenesis as they are considered an objective, non-invasive method for early detection of genetic damage.References: Mathers C, Boerma; T, Fat DM. The global burden of disease: 2004 update. Geneva, Switzerland: World Health organzation; 2008.
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Dev MS, Grover S, Batra J, Talathi R, Jaiswal M, Patil RS. Micronucleus as a Non-invasive Biomarker – A Review. J Adv Med Pharm Sci. 2016;6(4):1–9.
Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anomalies in buccal smears: Methods development. Mutat Res. 1992 Mar 1;271:69–77.
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