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Micropillar-assisted electric field enhancement for high-efficiency inactivation of bacteria
EP30258
Poster Title: Micropillar-assisted electric field enhancement for high-efficiency inactivation of bacteria
Submitted on 18 Jun 2019
Author(s): S Pudasaini · A. T. K. Perera · S. S. U. Ahmed · Sum Huan Ng · Chun Yang
Affiliations: School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798
This poster was presented at SelectBIO Lab-on-a-Chip and Microfluidics Europe 2019
Poster Views: 261
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Poster Information
Abstract: Development of high-efficiency and environment friendly bacterial inactivation methods is of great importance for preventing waterborne diseases which are one of the leading causes of death in the world. Traditional bacterial inactivation techniques have several limitations such as longer treatment time, use of harmful chemicals, formation of toxic byproducts, bacterial regrowth etc. Here, an electroporation-based continuous flow insulator-based AC dielectrophoresis device (iDEP) is developed and that the device achieved high bacterial inactivation performance (>99.9%) within a short exposure time (<1 s). Inactivation performance was evaluated for Escherichia coli and Enterococcus faecalis under various electric field conditions. More than 4 log removal of bacteria was obtained with an applied voltage of 300 V for the flowrate of 1 mL/hr. Images from scanning electron microscope confirmed the formation of electroporation-induced nano-pore within the cell membrane. The reported method of inactivation does not involve any chemicals and the formation of harmful by-products is also minimized. Summary: This paper presents an electroporation-based continuous flow insulator-based AC dielectrophoresis device (iDEP) that achieved high bacterial inactivation performance (>99.9%) within a short exposure time (<1 s). References: [1] Kale, A., Patel, S., Hu, G., Xuan, X., Electrophoresis 2013, 34, 674-683.
[2] Lewpiriyawong, N., Yang, C., Lam, Y. C., Electrophoresis 2010, 31, 2622-2631.
[3] Pudasaini S, Perera A. T. K., Ahmed S. S. U., Ng S. H., Yang, C., Electrophoresis 2019.
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