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MicroScale Thermophoresis - a versatile tool to study aptamer-target interactions beyond classical binding parameters
EP23887
Poster Title: MicroScale Thermophoresis - a versatile tool to study aptamer-target interactions beyond classical binding parameters
Submitted on 09 Mar 2016
Author(s): Clemens Entzian, Corinna Kuttenberger, Tobias Mauerer, Lukas Kniep, Estefanía Muciño, Dr. Thomas Schubert
Affiliations: 2bind GmbH
This poster was presented at 3rd Aptamers Oxford Congress, UK
Poster Views: 2,019
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Poster Information
Abstract: Aptamers are potent binding molecules recognizing various classes of target molecules. Thereby, aptamers are used in a great variety of different complex approaches in therapeutics and diagnostics. Establishment of these complex assays often need more information than just the classical binding parameters such as affinity, stoichiometry and thermodynamics. Detailed information on aptamer binding site, aptamer performance in complex liquids or with multiple binding partners may be necessary. In therapeutics, information on the potency of an aptamer to inhibit an important target interaction may be of great interest. The versatile technique of MicroScale Thermophoresis offers a great variety of different highly informative assays to satisfy the need for details in a highly sensitive cost- and time-efficient performance. This section describes different fast and precise MicroScale Thermophoresis assays such as competition assay, binding assay in complex liquids, assay with multiple binding partners and binding site mapping, that offer a great variety of information. Summary: Detailed information on aptamer binding site, aptamer performance in complex liquids or with multiple binding partners is indispensable for the information acquisition on the potency of an aptamer to inhibit an important target interaction. MicroScale Thermophoresis assays such as competition assay, binding assay in complex liquids, assay with multiple binding partners and binding site mapping offer a great variety of information.References: Alsager, Omar A.; Kumar, Shalen; Zhu, Bicheng; Travas-Sejdic, Jadranka; McNatty, Kenneth P.; Hodgkiss, Justin M. (2015): Ultrasensitive Colorimetric Detection of 17β-Estradiol. The Effect of Shortening DNA Aptamer Sequences. In Anal. Chem. 87 (8), pp. 4201–4209. DOI: 10.1021/acs.analchem.5b00335.

Breitsprecher, Dennis; Schlinck, Nina; Witte, David; Duhr, Stefan; Baaske, Philipp; Schubert, Thomas (2016): Aptamer Binding Studies Using MicroScale Thermophoresis. In Günter Mayer (Ed.): Nucleic acid aptamers. Selection, characterization, and application / edited by Günter Mayer, vol. 1380. New York, NY: Springer New York (Methods in Molecular Biology, 1380), pp. 99–111.

Durand, Guillaume; Lisi, Samuele; Ravelet, Corinne; Dausse, Eric; Peyrin, Eric; Toulmé, Jean-Jacques (2014): Riboswitches Based on Kissing Complexes for the Detection of Small Ligands. In Angew. Chem. Int. Ed. 53 (27), pp. 6942–6945. DOI: 10.1002/anie.201400402.

Entzian, Clemens; Schubert, Thomas
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