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Multiwell optogenetics for enhanced control of human iPSC-derived cells
EP25577
Poster Title: Multiwell optogenetics for enhanced control of human iPSC-derived cells
Submitted on 17 Feb 2017
Author(s): IP Clements, DC Millard, AM Nicolini, CA Arrowood, SA Chvatal, M Clements, JD Ross
Affiliations: Axion BioSystems
This poster was presented at Stem Cells in Drug Discovery 2017
Poster Views: 925
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Poster Information
Abstract: Optogenetic techniques enable precise optical manipulation of cells via integrated light-activated channels (opsins). Expression of light-sensitive proteins allows the user to activate or suppress activity in targeted cell types or selected biomolecular pathways. Pairing of optical stimulation with simultaneous microelectrode array (MEA) recordings provides a powerful tool for monitoring and manipulating cultured cell activity in vitro, offering insight into neuronal network interactions, stem cell characterization, toxicology screening, and drug safety and development. Here we explore various applications of Lumos, a commercial multiwell optical stimulation system, when paired with the Maestro, a system for high throughput MEA recording. In one case-study, human iPSC-derived cardiomyocytes were optically paced and challenged with test compounds in a Maestro MEA assay. Light-mediated pacing of hiPSC-cardiomyocyte activity was found to reduce well-to-well variability and allowed rate-dependent effects of test compounds to be explored [1]. These findings demonstrate how scaling in vitro optical stimulation and MEA-based recording to high well counts enables enhanced, high-throughput studies.Summary: Light-mediated pacing of hiPSC-cardiomyocyte activity was found to reduce well-to-well variability and allowed rate-dependent effects of test compounds to be explored. These findings demonstrate how scaling in vitro optical stimulation and MEA-based recording to high well counts enables enhanced, high-throughput studies.References: [1] Clements, I. P., Millard, D. C., Nicolini, A. M., Preyer, A. J., Grier, R., Heckerling, A., Blum, R. A., Tyler, P., McSweeney, K. M., Lub, Y. F., Hallb, D., and Ross, J. D. (2016). Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications. Proc. SPIE 9690Report abuse »
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